Stable integration of the Mrx1-roGFP2 biosensor to monitor dynamic changes of the mycothiol redox potential in Corynebacterium glutamicum.

Amino Acid Sequence Bacterial Proteins / genetics Biosensing Techniques Corynebacterium glutamicum / genetics Gene Expression Genes, Reporter Green Fluorescent Proteins / chemistry Hydrogen Peroxide / metabolism Intracellular Space / metabolism Models, Biological Models, Molecular Mutation Oxidation-Reduction Oxidative Stress Protein Conformation Reactive Oxygen Species / metabolism Recombinant Fusion Proteins / chemistry Structure-Activity Relationship
Corynebacterium glutamicum Mrx1-roGFP2 Mycothiol Mycothiol redox potential

Journal

Redox biology
ISSN: 2213-2317
Titre abrégé: Redox Biol
Pays: Netherlands
ID NLM: 101605639

Informations de publication

Date de publication:
01 2019
Historique:
received: 05 09 2018
revised: 08 11 2018
accepted: 15 11 2018
pubmed: 28 11 2018
medline: 22 3 2019
entrez: 28 11 2018
Statut: ppublish

Résumé

Mycothiol (MSH) functions as major low molecular weight (LMW) thiol in the industrially important Corynebacterium glutamicum. In this study, we genomically integrated an Mrx1-roGFP2 biosensor in C. glutamicum to measure dynamic changes of the MSH redox potential (E

Identifiants

DOI: 10.1016/j.redox.2018.11.012 PMID: 30481728 PMC: PMC6258114
pubmed: 30481728
pii: S2213-2317(18)30819-X
doi: 10.1016/j.redox.2018.11.012
pmc: PMC6258114
pii:
doi:

Substances chimiques

Bacterial Proteins 0
Reactive Oxygen Species 0
Recombinant Fusion Proteins 0
Green Fluorescent Proteins 147336-22-9
Hydrogen Peroxide BBX060AN9V

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

514-525

Subventions

Organisme : European Research Council
ID : 615585
Pays : International

Informations de copyright

Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.

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Auteurs

Quach Ngoc Tung (QN)

Freie Universität Berlin, Institute for Biology-Microbiology, D-14195 Berlin, Germany.

Vu Van Loi (VV)

Freie Universität Berlin, Institute for Biology-Microbiology, D-14195 Berlin, Germany.

Tobias Busche (T)

Freie Universität Berlin, Institute for Biology-Microbiology, D-14195 Berlin, Germany; Center for Biotechnology (CeBiTec), Universitätsstraße 25, D-33615 Bielefeld, Germany.

Andreas Nerlich (A)

Department of Internal Medicine/Infectious Diseases and Respiratory Medicine, Charité -Universitätsmedizin Berlin, D-10117 Berlin, Germany.

Maren Mieth (M)

Department of Internal Medicine/Infectious Diseases and Respiratory Medicine, Charité -Universitätsmedizin Berlin, D-10117 Berlin, Germany.

Johanna Milse (J)

Center for Biotechnology (CeBiTec), Universitätsstraße 25, D-33615 Bielefeld, Germany.

Jörn Kalinowski (J)

Center for Biotechnology (CeBiTec), Universitätsstraße 25, D-33615 Bielefeld, Germany.

Andreas C Hocke (AC)

Department of Internal Medicine/Infectious Diseases and Respiratory Medicine, Charité -Universitätsmedizin Berlin, D-10117 Berlin, Germany.

Haike Antelmann (H)

Freie Universität Berlin, Institute for Biology-Microbiology, D-14195 Berlin, Germany. Electronic address: haike.antelmann@fu-berlin.de.

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Classifications MeSH

questionsmedicales.fr › Sciences de l'information › Sources d'information › Services d'information › Documentation › Données de séquences moléculaires › Séquence d'acides aminés › Stable integration of the Mrx1-roGFP2...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Stable integration of the Mrx1-roGFP2...
questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Techniques de biocapteur › Stable integration of the Mrx1-roGFP2...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bâtonnets à Gram positif › Bâtonnets asporogènes à Gram positif › Bâtonnets asporogènes irréguliers à Gram positif › Corynebacterium › Corynebacterium glutamicum › Stable integration of the Mrx1-roGFP2...
questionsmedicales.fr › Phénomènes génétiques › Expression des gènes › Stable integration of the Mrx1-roGFP2...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Gènes › Gènes rapporteurs › Stable integration of the Mrx1-roGFP2...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines luminescentes › Protéines à fluorescence verte › Stable integration of the Mrx1-roGFP2...
questionsmedicales.fr › Composés chimiques organiques › Radicaux libres › Peroxydes › Peroxyde d'hydrogène › Stable integration of the Mrx1-roGFP2...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Stable integration of the Mrx1-roGFP2...
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles biologiques › Stable integration of the Mrx1-roGFP2...
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles moléculaires › Stable integration of the Mrx1-roGFP2...
questionsmedicales.fr › Phénomènes génétiques › Variation génétique › Mutation › Stable integration of the Mrx1-roGFP2...
questionsmedicales.fr › Métabolisme › Métabolisme énergétique › Oxydoréduction › Stable integration of the Mrx1-roGFP2...
questionsmedicales.fr › Phénomènes physiologiques › Stress physiologique › Stress oxydatif › Stable integration of the Mrx1-roGFP2...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Stable integration of the Mrx1-roGFP2...
questionsmedicales.fr › Produits chimiques inorganiques › Composés de l'oxygène › Espèces réactives de l'oxygène › Stable integration of the Mrx1-roGFP2...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines recombinantes › Protéines de fusion recombinantes › Stable integration of the Mrx1-roGFP2...
questionsmedicales.fr › Phénomènes physiologiques › Phénomènes pharmacologiques et toxicologiques › Phénomènes pharmacologiques › Relation structure-activité › Stable integration of the Mrx1-roGFP2...