Clonal analysis revealed functional heterogeneity in cancer stem-like cell phenotypes in uterine endometrioid adenocarcinoma.
Animals
Carboplatin
/ pharmacology
Carcinoma, Endometrioid
/ genetics
Clone Cells
/ pathology
Culture Media
DNA, Neoplasm
/ genetics
Endometrial Neoplasms
/ genetics
Female
Gene Expression Regulation, Neoplastic
Heterografts
Humans
Mice
Neoplastic Stem Cells
/ drug effects
Paclitaxel
/ pharmacology
Phenotype
Sequence Analysis, DNA
Serum
Spheroids, Cellular
Tumor Cells, Cultured
Cancer stem cell
Chemo-resistance
Endometrial carcinoma
SAGE-Seq
Tumorigenicity
Journal
Experimental and molecular pathology
ISSN: 1096-0945
Titre abrégé: Exp Mol Pathol
Pays: Netherlands
ID NLM: 0370711
Informations de publication
Date de publication:
02 2019
02 2019
Historique:
received:
02
10
2018
revised:
12
11
2018
accepted:
26
11
2018
pubmed:
7
12
2018
medline:
21
3
2019
entrez:
4
12
2018
Statut:
ppublish
Résumé
Uterine endometrial carcinoma is one of the common cancers in females. Cancer stem-like cells (CSCs)/cancer-initiating cells (CICs) are a small subpopulation of cancer cells that are tumorigenic and are resistant to treatments, thus they are focused as treatment targets. However, the heterogeneity of CSCs/CICs is still elusive, and we therefore analyzed CSCs/CICs at the clonal level. We previously established sphere-cultured CSCs/CICs from primary human uterine endometrial carcinoma, and we isolated several clones from CSCs/CICs in this study. Interestingly, we established two types of clones based on the growth pattern. The clones were termed sphere clones (S clones) and leukemia-like clones (LL clones). Functional analysis revealed that S clones are resistant to chemotherapy, whereas LL clones are sensitive to chemotherapy. On the other hand, S clones are less tumorigenic, while LL clones are highly tumorigenic. Transcriptome analysis using serial analysis of gene expression sequencing (SAGE-Seq) revealed distinctive gene expression profiles in S clone cells and LL clone cells. The results indicate that CSCs/CICs are composed of functionally heterogenic subpopulations including highly tumorigenic clones and treatment-resistant clones and that the characteristics of CSCs/CICs might be determined by the characteristics of different clones that compose CSCs/CICs.
Identifiants
pubmed: 30503404
pii: S0014-4800(18)30465-9
doi: 10.1016/j.yexmp.2018.11.013
pii:
doi:
Substances chimiques
Culture Media
0
DNA, Neoplasm
0
Carboplatin
BG3F62OND5
Paclitaxel
P88XT4IS4D
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
78-88Informations de copyright
Copyright © 2018. Published by Elsevier Inc.