Posttranscriptional spin labeling of RNA by tetrazine-based cycloaddition.

Click Chemistry Cycloaddition Reaction Electron Spin Resonance Spectroscopy Molecular Structure Nitrogen Oxides / chemistry Pyrroles / chemistry RNA / chemistry Spin Labels Tetrazoles / chemistry

Journal

Organic & biomolecular chemistry
ISSN: 1477-0539
Titre abrégé: Org Biomol Chem
Pays: England
ID NLM: 101154995

Informations de publication

Date de publication:
13 02 2019
Historique:
pubmed: 7 12 2018
medline: 4 4 2019
entrez: 7 12 2018
Statut: ppublish

Résumé

The site-specific introduction of spin labels into RNA for distance measurements by EPR gives insight into its solution structure. We here present a method for spin labeling of in vitro transcribed RNA. Distance distributions between two nitroxide spin labels are determined by PELDOR in a self-complementary RNA duplex.

Identifiants

DOI: 10.1039/c8ob02597e PMID: 30520916
pubmed: 30520916
doi: 10.1039/c8ob02597e
doi:

Substances chimiques

Nitrogen Oxides 0
Pyrroles 0
Spin Labels 0
Tetrazoles 0
pyrroline 28350-87-0
RNA 63231-63-0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1805-1808

Auteurs

Christof Domnick (C)

Life & Medical Sciences Institute, Chemical Biology & Medicinal Chemistry Unit, University of Bonn, Gerhard-Domagk-Str. 1, 53121 Bonn, Germany. skath@uni-bonn.de.

Gregor Hagelueken (G)

Frank Eggert (F)

Olav Schiemann (O)

Stephanie Kath-Schorr (S)

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Classifications MeSH

questionsmedicales.fr › Technologie, industrie et agriculture › Technologie › Technologie pharmaceutique › Techniques de chimie synthétique › Chimie click › Posttranscriptional spin labeling of RNA by...
questionsmedicales.fr › Technologie, industrie et agriculture › Technologie › Technologie pharmaceutique › Techniques de chimie synthétique › Réaction de cycloaddition › Posttranscriptional spin labeling of RNA by...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Analyse spectrale › Spectroscopie par résonance magnétique › Spectroscopie de résonance de spin électronique › Posttranscriptional spin labeling of RNA by...
questionsmedicales.fr › Phénomènes chimiques › Structure moléculaire › Posttranscriptional spin labeling of RNA by...
questionsmedicales.fr › Produits chimiques inorganiques › Composés de l'oxygène › Oxydes › Oxydes d'azote › Posttranscriptional spin labeling of RNA by...
questionsmedicales.fr › Composés hétérocycliques › Composés hétéromonocycliques › Azoles › Pyrroles › Posttranscriptional spin labeling of RNA by...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › Posttranscriptional spin labeling of RNA by...
questionsmedicales.fr › Composés chimiques organiques › Radicaux libres › Marqueurs de spin › Posttranscriptional spin labeling of RNA by...
questionsmedicales.fr › Composés hétérocycliques › Composés hétéromonocycliques › Azoles › Tétrazoles › Posttranscriptional spin labeling of RNA by...