RNA-seq Analysis of the SCN1A-KO Model based on CRISPR/Cas9 Genome Editing Technology.

Animals CRISPR-Cas Systems Cell Line Epilepsies, Myoclonic / genetics Gene Editing Gene Expression Gene Knockout Techniques / methods Mice NAV1.1 Voltage-Gated Sodium Channel / deficiency RNA, Messenger / metabolism Sequence Analysis, RNA Signal Transduction
CRISPR/Cas9 Dravet syndrome RNA-seq SCN1A epilepsy genomics

Journal

Neuroscience
ISSN: 1873-7544
Titre abrégé: Neuroscience
Pays: United States
ID NLM: 7605074

Informations de publication

Date de publication:
01 02 2019
Historique:
received: 20 06 2018
revised: 30 11 2018
accepted: 30 11 2018
pubmed: 12 12 2018
medline: 19 3 2019
entrez: 12 12 2018
Statut: ppublish

Résumé

Dravet syndrome (DS) is a disease that is primarily caused by the inactivation of the SCN1A-encoded voltage-gated sodium channel alpha subunit (Nav1.1). In this study, we constructed an SCN1A gene knockout model using CRISPR/Cas9 genome editing technology to deprive the Nav1.1 function in vitro. With mRNA-seq analysis we found abundant gene changes after SCN1A knockout, which associated with various signaling pathways, such as cancer pathways, the PI3K-AKT signaling pathway, the MAPK signaling pathway, and pathways involved in HTLV-I infection. We also noticed changes in the spliceosome, decreased glycolytic capacity, disturbances in calcium signaling pathways, and changes in the potassium, sodium, chloride, and calcium plasma channels after SCN1A knockout. In this study, we have been the first time to discover these changes and summarize them here and hope it would provide some clue for the study of Nav1.1 in the nervous system.

Identifiants

DOI: 10.1016/j.neuroscience.2018.11.052 PMID: 30529264
pubmed: 30529264
pii: S0306-4522(18)30794-2
doi: 10.1016/j.neuroscience.2018.11.052
pii:
doi:

Substances chimiques

NAV1.1 Voltage-Gated Sodium Channel 0
RNA, Messenger 0
Scn1a protein, mouse 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

1-11

Informations de copyright

Copyright © 2018 IBRO. Published by Elsevier Ltd. All rights reserved.

Auteurs

Xiaoguang Shi (X)

The Ningxia Key Laboratory of Cerebrocranial Diseases, Incubation Base of the National Key Laboratory, Ningxia Medical University, Yinchuan, China.

Wenxin He (W)

The Ningxia Key Laboratory of Cerebrocranial Diseases, Incubation Base of the National Key Laboratory, Ningxia Medical University, Yinchuan, China.

Shanshan Guo (S)

The Ningxia Key Laboratory of Cerebrocranial Diseases, Incubation Base of the National Key Laboratory, Ningxia Medical University, Yinchuan, China.

Bingying Zhang (B)

Department of Biochemistry and Molecular Biology, The School of Basic Medical Sciences, Ningxia Medical University, Yinchuan, China.

Shuanglai Ren (S)

The Ningxia Key Laboratory of Cerebrocranial Diseases, Incubation Base of the National Key Laboratory, Ningxia Medical University, Yinchuan, China.

Kunmei Liu (K)

The Ningxia Key Laboratory of Cerebrocranial Diseases, Incubation Base of the National Key Laboratory, Ningxia Medical University, Yinchuan, China.

Tao Sun (T)

The Ningxia Key Laboratory of Cerebrocranial Diseases, Incubation Base of the National Key Laboratory, Ningxia Medical University, Yinchuan, China.

Jianqi Cui (J)

The Ningxia Key Laboratory of Cerebrocranial Diseases, Incubation Base of the National Key Laboratory, Ningxia Medical University, Yinchuan, China; Department of Biochemistry and Molecular Biology, The School of Basic Medical Sciences, Ningxia Medical University, Yinchuan, China. Electronic address: Jianqi@gmail.com.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › RNA-seq Analysis of the SCN1A-KO Model based...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Épigenèse génétique › Extinction de l'expression des gènes › Systèmes CRISPR-Cas › RNA-seq Analysis of the SCN1A-KO Model based...
questionsmedicales.fr › Cellules › Cellules cultivées › Lignée cellulaire › RNA-seq Analysis of the SCN1A-KO Model based...
questionsmedicales.fr › Maladies du système nerveux › Maladies du système nerveux central › Encéphalopathies › Épilepsie › Syndromes épileptiques › Épilepsies myocloniques › RNA-seq Analysis of the SCN1A-KO Model based...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Génie génétique › Édition de gène › RNA-seq Analysis of the SCN1A-KO Model based...
questionsmedicales.fr › Phénomènes génétiques › Expression des gènes › RNA-seq Analysis of the SCN1A-KO Model based...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Ciblage de gène › Techniques de knock-out de gènes › RNA-seq Analysis of the SCN1A-KO Model based...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › RNA-seq Analysis of the SCN1A-KO Model based...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines de tissu nerveux › Canaux sodiques voltage-dépendants › Canal sodique voltage-dépendant NAV1.1 › RNA-seq Analysis of the SCN1A-KO Model based...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN messager › RNA-seq Analysis of the SCN1A-KO Model based...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Analyse de séquence › Analyse de séquence d'ARN › RNA-seq Analysis of the SCN1A-KO Model based...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Transduction du signal › RNA-seq Analysis of the SCN1A-KO Model based...