A Phytophthora Effector Suppresses Trans-Kingdom RNAi to Promote Disease Susceptibility.

Arabidopsis / genetics Arabidopsis Proteins / genetics Disease Susceptibility / microbiology Gene Expression Regulation, Plant Gene Silencing Genes, Reporter / genetics Host-Pathogen Interactions / genetics MicroRNAs / genetics Phosphoprotein Phosphatases / antagonists & inhibitors Phytophthora / metabolism Plant Diseases / immunology Plant Immunity / genetics Plant Leaves / immunology RNA Interference / immunology RNA, Small Interfering / biosynthesis RNA-Binding Proteins / genetics Nicotiana Verticillium Virulence Virulence Factors / genetics
RNA interference RNA-silencing suppressor host-induced gene silencing microRNA pathogenesis plant immunity secondary small RNAs virulence factor

Journal

Cell host & microbe
ISSN: 1934-6069
Titre abrégé: Cell Host Microbe
Pays: United States
ID NLM: 101302316

Informations de publication

Date de publication:
09 01 2019
Historique:
received: 08 07 2018
revised: 03 10 2018
accepted: 22 10 2018
pubmed: 1 1 2019
medline: 15 3 2019
entrez: 1 1 2019
Statut: ppublish

Résumé

RNA silencing (RNAi) has a well-established role in anti-viral immunity in plants. The destructive eukaryotic pathogen Phytophthora encodes suppressors of RNAi (PSRs), which enhance plant susceptibility. However, the role of small RNAs in defense against eukaryotic pathogens is unclear. Here, we show that Phytophthora infection of Arabidopsis leads to increased production of a diverse pool of secondary small interfering RNAs (siRNAs). Instead of regulating endogenous plant genes, these siRNAs are found in extracellular vesicles and likely silence target genes in Phytophthora during natural infection. Introduction of a plant siRNA in Phytophthora leads to developmental deficiency and abolishes virulence, while Arabidopsis mutants defective in secondary siRNA biogenesis are hypersusceptible. Notably, Phytophthora effector PSR2 specifically inhibits secondary siRNA biogenesis in Arabidopsis and promotes infection. These findings uncover the role of siRNAs as antimicrobial agents against eukaryotic pathogens and highlight a defense/counter-defense arms race centered on trans-kingdom gene silencing between hosts and pathogens.

Identifiants

DOI: 10.1016/j.chom.2018.11.007 PMID: 30595554 PMC: PMC9208300
pubmed: 30595554
pii: S1931-3128(18)30589-4
doi: 10.1016/j.chom.2018.11.007
pmc: PMC9208300
mid: NIHMS1814613
pii:
doi:

Substances chimiques

Arabidopsis Proteins 0
DRB4 protein, Arabidopsis 0
MicroRNAs 0
RNA, Small Interfering 0
RNA-Binding Proteins 0
Virulence Factors 0
pentatricopeptide repeat protein, Arabidopsis 0
Phosphoprotein Phosphatases EC 3.1.3.16

Types de publication

Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

153-165.e5

Subventions

Organisme : NIGMS NIH HHS
ID : R01 GM129373
Pays : United States

Commentaires et corrections

Type : CommentIn

Informations de copyright

Copyright © 2018 Elsevier Inc. All rights reserved.

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Auteurs

Yingnan Hou (Y)

Department of Microbiology and Plant Pathology, University of California, Riverside, Riverside, CA 92521, USA; Center for Plant Cell Biology, University of California, Riverside, Riverside, CA 92521, USA.

Yi Zhai (Y)

Department of Microbiology and Plant Pathology, University of California, Riverside, Riverside, CA 92521, USA; Center for Plant Cell Biology, University of California, Riverside, Riverside, CA 92521, USA.

Li Feng (L)

Institute of Plant and Food Science, Department of Biology, Southern University of Science and Technology, Shenzhen 518055, China.

Hana Z Karimi (HZ)

Department of Biology, Indiana University, Bloomington, IN 47405, USA.

Brian D Rutter (BD)

Department of Biology, Indiana University, Bloomington, IN 47405, USA.

Liping Zeng (L)

Department of Microbiology and Plant Pathology, University of California, Riverside, Riverside, CA 92521, USA; Center for Plant Cell Biology, University of California, Riverside, Riverside, CA 92521, USA.

Du Seok Choi (DS)

Department of Microbiology and Plant Pathology, University of California, Riverside, Riverside, CA 92521, USA; Center for Plant Cell Biology, University of California, Riverside, Riverside, CA 92521, USA.

Bailong Zhang (B)

Center for Plant Cell Biology, University of California, Riverside, Riverside, CA 92521, USA; Department of Botany and Plant Science, University of California, Riverside, Riverside, CA 92521, USA.

Weifeng Gu (W)

Department of Cell Biology and Neuroscience, University of California, Riverside, Riverside, CA 92521, USA.

Xuemei Chen (X)

Center for Plant Cell Biology, University of California, Riverside, Riverside, CA 92521, USA; Department of Botany and Plant Science, University of California, Riverside, Riverside, CA 92521, USA.

Wenwu Ye (W)

Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.

Roger W Innes (RW)

Department of Biology, Indiana University, Bloomington, IN 47405, USA.

Jixian Zhai (J)

Institute of Plant and Food Science, Department of Biology, Southern University of Science and Technology, Shenzhen 518055, China.

Wenbo Ma (W)

Department of Microbiology and Plant Pathology, University of California, Riverside, Riverside, CA 92521, USA; Center for Plant Cell Biology, University of California, Riverside, Riverside, CA 92521, USA. Electronic address: wenbo.ma@ucr.edu.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Brassicaceae › Arabidopsis › A Phytophthora Effector Suppresses...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines végétales › Protéines d'Arabidopsis › A Phytophthora Effector Suppresses...
questionsmedicales.fr › Phénomènes physiologiques › Constitution physique › Prédisposition aux maladies › A Phytophthora Effector Suppresses...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Régulation de l'expression des gènes végétaux › A Phytophthora Effector Suppresses...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Épigenèse génétique › Extinction de l'expression des gènes › A Phytophthora Effector Suppresses...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Gènes › Gènes rapporteurs › A Phytophthora Effector Suppresses...
questionsmedicales.fr › Phénomènes biologiques › Interactions hôte-microbes › Interactions hôte-pathogène › A Phytophthora Effector Suppresses...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN non traduit › Petit ARN non traduit › microARN › A Phytophthora Effector Suppresses...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Phosphoprotein Phosphatases › A Phytophthora Effector Suppresses...
questionsmedicales.fr › Eucaryotes › Straménopiles › Oomycetes › Phytophthora › A Phytophthora Effector Suppresses...
questionsmedicales.fr › Phénomènes physiologiques des plantes › Maladies des plantes › A Phytophthora Effector Suppresses...
questionsmedicales.fr › Phénomènes physiologiques des plantes › Immunité des plantes › A Phytophthora Effector Suppresses...
questionsmedicales.fr › Structures de plante › Parties aériennes de plante › Feuilles de plante › A Phytophthora Effector Suppresses...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Épigenèse génétique › Extinction de l'expression des gènes › Interférence par ARN › A Phytophthora Effector Suppresses...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN non traduit › Petit ARN non traduit › Petit ARN interférent › A Phytophthora Effector Suppresses...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Nucléoprotéines › Protéines de liaison à l'ARN › A Phytophthora Effector Suppresses...
questionsmedicales.fr › Eucaryotes › Champignons › Deuteromycota › Verticillium › A Phytophthora Effector Suppresses...
questionsmedicales.fr › Phénomènes microbiologiques › Virulence › A Phytophthora Effector Suppresses...
questionsmedicales.fr › Facteurs biologiques › Toxines biologiques › Facteurs de virulence › A Phytophthora Effector Suppresses...