Agarose Gel Electrophoresis.

DNA / analysis Electrophoresis, Agar Gel / methods Fluorescent Dyes / metabolism Staining and Labeling / methods

Journal

Cold Spring Harbor protocols
ISSN: 1559-6095
Titre abrégé: Cold Spring Harb Protoc
Pays: United States
ID NLM: 101524530

Informations de publication

Date de publication:
02 01 2019
Historique:
entrez: 4 1 2019
pubmed: 4 1 2019
medline: 31 1 2020
Statut: epublish

Résumé

This protocol describes steps for the preparation and running of agarose gels and for staining and visualization of DNA in gels using three dyes: ethidium bromide, SYBR Gold, and SYBR Green 1.

Identifiants

DOI: 10.1101/pdb.prot100404 PMID: 30602560
pubmed: 30602560
pii: 2019/1/pdb.prot100404
doi: 10.1101/pdb.prot100404
doi:

Substances chimiques

Fluorescent Dyes 0
DNA 9007-49-2

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

© 2019 Cold Spring Harbor Laboratory Press.

Auteurs

Michael R Green (MR)

Joseph Sambrook (J)

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Classifications MeSH

questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › Agarose Gel Electrophoresis.
questionsmedicales.fr › Techniques d'investigation › Techniques électrochimiques › Électrophorèse › Électrophorèse sur gel d'agar › Agarose Gel Electrophoresis.
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Produits chimiques de laboratoire › Indicateurs et réactifs › Luminescents › Colorants fluorescents › Agarose Gel Electrophoresis.
questionsmedicales.fr › Techniques d'investigation › Techniques de laboratoire clinique › Techniques histologiques › Techniques de préparation histocytologique › Coloration et marquage › Agarose Gel Electrophoresis.