Phenotypic and molecular characterization of Escherichia albertii: Further surrogates to avoid potential laboratory misidentification.
Adhesins, Bacterial
/ genetics
Animals
Bacterial Toxins
/ genetics
Bacterial Typing Techniques
Bacteriological Techniques
/ methods
Enterobacteriaceae Infections
/ microbiology
Environmental Microbiology
Escherichia
/ classification
Escherichia coli Proteins
/ genetics
Food Microbiology
Humans
Multilocus Sequence Typing
Polymerase Chain Reaction
CDT
E. coli cytolethal distending toxin-II
EPEC
Escherichia albertii
eae
Journal
International journal of medical microbiology : IJMM
ISSN: 1618-0607
Titre abrégé: Int J Med Microbiol
Pays: Germany
ID NLM: 100898849
Informations de publication
Date de publication:
Mar 2019
Mar 2019
Historique:
received:
25
10
2018
revised:
16
12
2018
accepted:
28
12
2018
pubmed:
5
1
2019
medline:
14
6
2019
entrez:
5
1
2019
Statut:
ppublish
Résumé
Escherichia albertii is an emerging gastrointestinal pathogen, related to Escherichia coli, which can be misidentified as enteropathogenic E. coli (EPEC) and enterohemorrhagic E. coli (EHEC), due to the presence of the eae gene in E. albertii. The aim of this study was to verify our hypothesis that E. coli cytolethal distending toxin-II (Eccdt-II) gene-positive E. coli is E. albertii and to accumulate the data regarding the bacteriological characteristics of E. albertii. For these purposes, we attempted to detect E. albertii in eae gene-positive bacteria previously identified as E. coli and to examine if re-identified E. albertii contained Eccdt-II-homologous gene and remaining eae gene-positive E. coli did not. A total of 373 eae gene-positive E. coli strains were analyzed by biochemical tests, multilocus sequence analysis and an E. albertii-specific PCR. The strains re-identified as E. albertii were also examined for the presence of cdt genes by using
Identifiants
pubmed: 30606690
pii: S1438-4221(18)30541-1
doi: 10.1016/j.ijmm.2018.12.003
pii:
doi:
Substances chimiques
Adhesins, Bacterial
0
Bacterial Toxins
0
Escherichia coli Proteins
0
cytolethal distending toxin
0
eaeA protein, E coli
147094-99-3
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
108-115Informations de copyright
Copyright © 2018 Elsevier GmbH. All rights reserved.