Generation of a Broadly Cross-Neutralizing Antibody Fragment against Several Mexican Scorpion Venoms.
cross-neutralization
directed evolution
phage display
polyvalent antivenom
scFv
scorpion
Journal
Toxins
ISSN: 2072-6651
Titre abrégé: Toxins (Basel)
Pays: Switzerland
ID NLM: 101530765
Informations de publication
Date de publication:
10 01 2019
10 01 2019
Historique:
received:
11
12
2018
revised:
03
01
2019
accepted:
03
01
2019
entrez:
13
1
2019
pubmed:
13
1
2019
medline:
28
12
2019
Statut:
epublish
Résumé
The recombinant antibody fragments generated against the toxic components of scorpion venoms are considered a promising alternative for obtaining new antivenoms for therapy. Using directed evolution and site-directed mutagenesis, it was possible to generate a human single-chain antibody fragment with a broad cross-reactivity that retained recognition for its original antigen. This variant is the first antibody fragment that neutralizes the effect of an estimated 13 neurotoxins present in the venom of nine species of Mexican scorpions. This single antibody fragment showed the properties of a polyvalent antivenom. These results represent a significant advance in the development of new antivenoms against scorpion stings, since the number of components would be minimized due to their broad cross-neutralization capacity, while at the same time bypassing animal immunization.
Identifiants
pubmed: 30634620
pii: toxins11010032
doi: 10.3390/toxins11010032
pmc: PMC6356842
pii:
doi:
Substances chimiques
Antibodies, Neutralizing
0
Neurotoxins
0
Scorpion Venoms
0
Single-Chain Antibodies
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
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