Conformational changes and catalytic inefficiency associated with Mot1-mediated TBP-DNA dissociation.
Adenosine Triphosphatases
/ chemistry
Catalysis
DNA, Fungal
/ chemistry
Escherichia coli
Gene Expression Regulation, Fungal
Models, Molecular
Nucleic Acid Conformation
Promoter Regions, Genetic
Protein Binding
Protein Conformation
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
/ chemistry
TATA-Binding Protein Associated Factors
/ chemistry
TATA-Box Binding Protein
/ chemistry
Journal
Nucleic acids research
ISSN: 1362-4962
Titre abrégé: Nucleic Acids Res
Pays: England
ID NLM: 0411011
Informations de publication
Date de publication:
08 04 2019
08 04 2019
Historique:
accepted:
07
01
2019
revised:
21
12
2018
received:
07
02
2018
pubmed:
17
1
2019
medline:
13
11
2019
entrez:
17
1
2019
Statut:
ppublish
Résumé
The TATA-box Binding Protein (TBP) plays a central role in regulating gene expression and is the first step in the process of pre-initiation complex (PIC) formation on promoter DNA. The lifetime of TBP at the promoter site is controlled by several cofactors including the Modifier of transcription 1 (Mot1), an essential TBP-associated ATPase. Based on ensemble measurements, Mot1 can use adenosine triphosphate (ATP) hydrolysis to displace TBP from DNA and various models for how this activity is coupled to transcriptional regulation have been proposed. However, the underlying molecular mechanism of Mot1 action is not well understood. In this work, the interaction of Mot1 with the DNA/TBP complex was investigated by single-pair Förster resonance energy transfer (spFRET). Upon Mot1 binding to the DNA/TBP complex, a transition in the DNA/TBP conformation was observed. Hydrolysis of ATP by Mot1 led to a conformational change but was not sufficient to efficiently disrupt the complex. SpFRET measurements of dual-labeled DNA suggest that Mot1's ATPase activity primes incorrectly oriented TBP for dissociation from DNA and additional Mot1 in solution is necessary for TBP unbinding. These findings provide a framework for understanding how the efficiency of Mot1's catalytic activity is tuned to establish a dynamic pool of TBP without interfering with stable and functional TBP-containing complexes.
Identifiants
pubmed: 30649478
pii: 5289489
doi: 10.1093/nar/gky1322
pmc: PMC6451094
doi:
Substances chimiques
DNA, Fungal
0
Saccharomyces cerevisiae Proteins
0
TATA-Binding Protein Associated Factors
0
TATA-Box Binding Protein
0
Adenosine Triphosphatases
EC 3.6.1.-
MOT1 protein, S cerevisiae
EC 3.6.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2793-2806Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM055763
Pays : United States
Informations de copyright
© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.
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