A novel bioreactor for combined magnetic resonance spectroscopy and optical imaging of metabolism in 3D cell cultures.
Animals
Bioreactors
Cell Line, Tumor
Cell Survival
Collagen
/ chemistry
Contrast Media
Diffusion
Disease Progression
Equipment Design
Female
Gels
Glucose
/ metabolism
Lactic Acid
/ metabolism
Magnetic Resonance Spectroscopy
Mammary Neoplasms, Animal
/ diagnostic imaging
Mammary Neoplasms, Experimental
/ diagnostic imaging
Mice
NAD
/ pharmacology
Optical Imaging
Printing, Three-Dimensional
Pyruvic Acid
/ chemistry
Temperature
bioreactor
fluorescence lifetime imaging (FLIM)
lactate production
magnetic resonance spectroscopy (MRS)
metabolism
multimodal
nicotinamide adenine dinucleotide (NADH)
optical imaging
Journal
Magnetic resonance in medicine
ISSN: 1522-2594
Titre abrégé: Magn Reson Med
Pays: United States
ID NLM: 8505245
Informations de publication
Date de publication:
05 2019
05 2019
Historique:
received:
10
06
2018
revised:
28
11
2018
accepted:
28
11
2018
pubmed:
18
1
2019
medline:
25
3
2020
entrez:
18
1
2019
Statut:
ppublish
Résumé
Fluorescence lifetime imaging microscopy (FLIM) of endogenous fluorescent metabolites permits the measurement of cellular metabolism in cell, tissue and animal models. In parallel, magnetic resonance spectroscopy (MRS) of dynamic nuclear (hyper)polarized (DNP) The model fitting for FLIM data analysis and the theory behind a model for the diffusion of pyruvate into a collagen gel are detailed. The device is MRI-compatible, including an optical window, a temperature control system and an injection port for the introduction of contrast agents. Three-dimensional printing, computer numerical control machining and laser cutting were used to fabricate custom parts. Performance of the bioreactor is demonstrated for 4 T1 murine breast cancer cells under glucose deprivation. Mean nicotinamide adenine dinucleotide (NADH) fluorescence lifetimes were 10% longer and hyperpolarized A novel bioreactor that is compatible with, and can exploit the benefits of, both FLIM and
Identifiants
pubmed: 30652350
doi: 10.1002/mrm.27644
pmc: PMC6414270
mid: NIHMS1000763
doi:
Substances chimiques
Contrast Media
0
Gels
0
NAD
0U46U6E8UK
Lactic Acid
33X04XA5AT
Pyruvic Acid
8558G7RUTR
Collagen
9007-34-5
Glucose
IY9XDZ35W2
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
3379-3391Subventions
Organisme : NCATS NIH HHS
ID : TL1 TR002375
Pays : United States
Organisme : NCI NIH HHS
ID : T32 CA009206
Pays : United States
Organisme : NCATS NIH HHS
ID : TL1 TR000429
Pays : United States
Organisme : NIH/NCI
ID : T32CA00926
Pays : International
Organisme : NIH HHS
ID : CA142833
Pays : United States
Organisme : NIH/NCATS
ID : UL1TR000427 and TL1TR000429
Pays : International
Organisme : NCATS NIH HHS
ID : UL1 TR002373
Pays : United States
Organisme : NIH/NCATS
ID : TL1TR000429
Pays : International
Organisme : NCI NIH HHS
ID : R01 CA185251
Pays : United States
Organisme : NIH/NCATS
ID : UL1TR000427
Pays : International
Organisme : NHLBI NIH HHS
ID : R01 HL126771
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA179556
Pays : United States
Organisme : NIH HHS
ID : CA185251
Pays : United States
Organisme : NCATS NIH HHS
ID : UL1 TR000427
Pays : United States
Organisme : NIH HHS
ID : CA142833, CA185251 and CA179556.
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA142833
Pays : United States
Organisme : NIH HHS
ID : CA179556.
Pays : United States
Informations de copyright
© 2019 International Society for Magnetic Resonance in Medicine.
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