Sucrose Metabolism in Haloarchaea: Reassessment Using Genomics, Proteomics, and Metagenomics.


Journal

Applied and environmental microbiology
ISSN: 1098-5336
Titre abrégé: Appl Environ Microbiol
Pays: United States
ID NLM: 7605801

Informations de publication

Date de publication:
15 03 2019
Historique:
received: 11 12 2018
accepted: 10 01 2019
pubmed: 20 1 2019
medline: 28 2 2020
entrez: 20 1 2019
Statut: epublish

Résumé

The canonical pathway for sucrose metabolism in haloarchaea utilizes a modified Embden-Meyerhof-Parnas pathway (EMP), in which ketohexokinase and 1-phosphofructokinase phosphorylate fructose released from sucrose hydrolysis. However, our survey of haloarchaeal genomes determined that ketohexokinase and 1-phosphofructokinase genes were not present in all species known to utilize fructose and sucrose, thereby indicating that alternative mechanisms exist for fructose metabolism. A fructokinase gene was identified in the majority of fructose- and sucrose-utilizing species, whereas only a small number possessed a ketohexokinase gene. Analysis of a range of hypersaline metagenomes revealed that haloarchaeal fructokinase genes were far more abundant (37 times) than haloarchaeal ketohexokinase genes. We used proteomic analysis of

Identifiants

pubmed: 30658981
pii: AEM.02935-18
doi: 10.1128/AEM.02935-18
pmc: PMC6414365
pii:
doi:

Substances chimiques

Archaeal Proteins 0
Sucrose 57-50-1
Fructokinases EC 2.7.1.-
Phosphofructokinase-1 EC 2.7.1.11
ketohexokinase EC 2.7.1.3
fructokinase EC 2.7.1.4
1-phosphofructokinase EC 2.7.1.56

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

Copyright © 2019 American Society for Microbiology.

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Auteurs

Timothy J Williams (TJ)

School of Biotechnology and Biomolecular Sciences, University of New South Wales Sydney, Sydney, New South Wales, Australia.

Michelle A Allen (MA)

School of Biotechnology and Biomolecular Sciences, University of New South Wales Sydney, Sydney, New South Wales, Australia.

Yan Liao (Y)

School of Biotechnology and Biomolecular Sciences, University of New South Wales Sydney, Sydney, New South Wales, Australia.

Mark J Raftery (MJ)

Bioanalytical Mass Spectrometry Facility, University of New South Wales Sydney, Sydney, New South Wales, Australia.

Ricardo Cavicchioli (R)

School of Biotechnology and Biomolecular Sciences, University of New South Wales Sydney, Sydney, New South Wales, Australia r.cavicchioli@unsw.edu.au.

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Classifications MeSH