A brain-wide functional map of the serotonergic responses to acute stress and fluoxetine.
Animals
Brain Mapping
/ methods
Cerebral Cortex
/ diagnostic imaging
Cerebrovascular Circulation
/ drug effects
Dorsal Raphe Nucleus
/ diagnostic imaging
Evoked Potentials, Visual
/ drug effects
Female
Fluoxetine
/ pharmacology
Immobilization
Magnetic Resonance Imaging
Male
Mice
Mice, Transgenic
Optogenetics
Photic Stimulation
Receptors, Serotonin
/ metabolism
Serotonergic Neurons
/ drug effects
Serotonin
/ metabolism
Selective Serotonin Reuptake Inhibitors
/ pharmacology
Stress, Psychological
/ metabolism
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
21 01 2019
21 01 2019
Historique:
received:
24
04
2018
accepted:
26
12
2018
entrez:
22
1
2019
pubmed:
22
1
2019
medline:
6
2
2019
Statut:
epublish
Résumé
Central serotonin (5-HT) orchestrates myriad cognitive processes and lies at the core of many stress-related psychiatric illnesses. However, the basic relationship between its brain-wide axonal projections and functional dynamics is not known. Here we combine optogenetics and fMRI to produce a brain-wide 5-HT evoked functional map. We find that DRN photostimulation leads to an increase in the hemodynamic response in the DRN itself, while projection areas predominately exhibit a reduction of cerebral blood volume mirrored by suppression of cortical delta oscillations. We find that the regional distribution of post-synaptically expressed 5-HT receptors better correlates with DRN 5-HT functional connectivity than anatomical projections. Our work suggests that neuroarchitecture is not the primary determinant of function for the DRN 5-HT. With respect to two 5-HT elevating stimuli, we find that acute stress leads to circuit-wide blunting of the DRN output, while the SSRI fluoxetine noticeably enhances DRN functional connectivity. These data provide fundamental insight into the brain-wide functional dynamics of the 5-HT projection system.
Identifiants
pubmed: 30664643
doi: 10.1038/s41467-018-08256-w
pii: 10.1038/s41467-018-08256-w
pmc: PMC6341094
doi:
Substances chimiques
Receptors, Serotonin
0
Serotonin Uptake Inhibitors
0
Fluoxetine
01K63SUP8D
Serotonin
333DO1RDJY
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
350Commentaires et corrections
Type : ErratumIn
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