Progress and Challenges for Live-cell Imaging of Genomic Loci Using CRISPR-based Platforms.

CRISPR-Cas Systems / genetics Clustered Regularly Interspaced Short Palindromic Repeats / genetics Genetic Loci Genomics Molecular Imaging / methods Nanoparticles / chemistry
CRISPR Cas9 Genomic imaging dCas9 sgRNA

Journal

Genomics, proteomics & bioinformatics
ISSN: 2210-3244
Titre abrégé: Genomics Proteomics Bioinformatics
Pays: China
ID NLM: 101197608

Informations de publication

Date de publication:
04 2019
Historique:
received: 12 06 2018
revised: 11 10 2018
accepted: 31 10 2018
pubmed: 3 2 2019
medline: 18 10 2019
entrez: 3 2 2019
Statut: ppublish

Résumé

Chromatin conformation, localization, and dynamics are crucial regulators of cellular behaviors. Although fluorescence in situ hybridization-based techniques have been widely utilized for investigating chromatin architectures in healthy and diseased states, the requirement for cell fixation precludes the comprehensive dynamic analysis necessary to fully understand chromatin activities. This has spurred the development and application of a variety of imaging methodologies for visualizing single chromosomal loci in the native cellular context. In this review, we describe currently-available approaches for imaging single genomic loci in cells, with special focus on clustered regularly interspaced short palindromic repeats (CRISPR)-based imaging approaches. In addition, we discuss some of the challenges that limit the application of CRISPR-based genomic imaging approaches, and potential solutions to address these challenges. We anticipate that, with continued refinement of CRISPR-based imaging techniques, significant understanding can be gained to help decipher chromatin activities and their relevance to cellular physiology and pathogenesis.

Identifiants

DOI: 10.1016/j.gpb.2018.10.001 PMID: 30710789 PMC: PMC6620262
pubmed: 30710789
pii: S1672-0229(18)30158-X
doi: 10.1016/j.gpb.2018.10.001
pmc: PMC6620262
pii:
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't Review

Langues

eng

Sous-ensembles de citation

IM

Pagination

119-128

Informations de copyright

Copyright © 2019 The Authors. Production and hosting by Elsevier B.V. All rights reserved.

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Auteurs

Xiaotian Wu (X)

Department of Biomedical Engineering, College of Engineering, Peking University, Beijing 100871, China; School of Life Sciences, Peking University, Beijing 100871, China.

Shiqi Mao (S)

Department of Biomedical Engineering, College of Engineering, Peking University, Beijing 100871, China.

Yachen Ying (Y)

Department of Biomedical Engineering, College of Engineering, Peking University, Beijing 100871, China.

Christopher J Krueger (CJ)

Department of Biomedical Engineering, College of Engineering, Peking University, Beijing 100871, China; Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA.

Antony K Chen (AK)

Department of Biomedical Engineering, College of Engineering, Peking University, Beijing 100871, China. Electronic address: chenak@pku.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Épigenèse génétique › Extinction de l'expression des gènes › Systèmes CRISPR-Cas › Progress and Challenges for Live-cell Imaging...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Séquences répétées en tandem › Clustered regularly interspaced short palindromic repeats › Progress and Challenges for Live-cell Imaging...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Locus génétiques › Progress and Challenges for Live-cell Imaging...
questionsmedicales.fr › Disciplines des sciences naturelles › Disciplines des sciences biologiques › Biologie › Génétique › Génomique › Progress and Challenges for Live-cell Imaging...
questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Imagerie moléculaire › Progress and Challenges for Live-cell Imaging...
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Nanostructures › Nanoparticules › Progress and Challenges for Live-cell Imaging...