On the Mechanism and Origin of Isoleucyl-tRNA Synthetase Editing against Norvaline.

Escherichia coli / genetics Escherichia coli Proteins / genetics Isoleucine-tRNA Ligase / genetics Protein Biosynthesis RNA Editing Valine / analogs & derivatives
aminoacyl-tRNA synthetase mistranslation non-proteinogenic amino acids primordial translation proofreading

Journal

Journal of molecular biology
ISSN: 1089-8638
Titre abrégé: J Mol Biol
Pays: Netherlands
ID NLM: 2985088R

Informations de publication

Date de publication:
15 03 2019
Historique:
received: 16 12 2018
revised: 20 01 2019
accepted: 22 01 2019
pubmed: 4 2 2019
medline: 24 3 2020
entrez: 4 2 2019
Statut: ppublish

Résumé

Aminoacyl-tRNA synthetases (aaRSs), the enzymes responsible for coupling tRNAs to their cognate amino acids, minimize translational errors by intrinsic hydrolytic editing. Here, we compared norvaline (Nva), a linear amino acid not coded for protein synthesis, to the proteinogenic, branched valine (Val) in their propensity to mistranslate isoleucine (Ile) in proteins. We show that in the synthetic site of isoleucyl-tRNA synthetase (IleRS), Nva and Val are activated and transferred to tRNA at similar rates. The efficiency of the synthetic site in pre-transfer editing of Nva and Val also appears to be similar. Post-transfer editing was, however, more rapid with Nva and consequently IleRS misaminoacylates Nva-tRNA

Identifiants

DOI: 10.1016/j.jmb.2019.01.029 PMID: 30711543
pubmed: 30711543
pii: S0022-2836(19)30046-4
doi: 10.1016/j.jmb.2019.01.029
pii:
doi:

Substances chimiques

Escherichia coli Proteins 0
norvaline A70UKS48FE
Isoleucine-tRNA Ligase EC 6.1.1.5
Valine HG18B9YRS7

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1284-1297

Informations de copyright

Copyright © 2019 Elsevier Ltd. All rights reserved.

Auteurs

Mirna Bilus (M)

Department of Chemistry, Faculty of Science, University of Zagreb, Zagreb 10000, Croatia.

Maja Semanjski (M)

Proteome Center Tuebingen, University of Tuebingen, Tuebingen 72076, Germany.

Marko Mocibob (M)

Department of Chemistry, Faculty of Science, University of Zagreb, Zagreb 10000, Croatia.

Igor Zivkovic (I)

Department of Chemistry, Faculty of Science, University of Zagreb, Zagreb 10000, Croatia.

Nevena Cvetesic (N)

Institute of Clinical Sciences, Faculty of Medicine, Imperial College London, and the MRC London Institute of Medical Sciences, London, W12 0NN, United Kingdom.

Dan S Tawfik (DS)

Department of Biomolecular Sciences, Weizmann Institute of Science, Rehovot 76100, Israel.

Agnes Toth-Petroczy (A)

Max Planck Institute of Molecular Cell Biology and Genetics, Dresden 01307, Germany.

Boris Macek (B)

Proteome Center Tuebingen, University of Tuebingen, Tuebingen 72076, Germany.

Ita Gruic-Sovulj (I)

Department of Chemistry, Faculty of Science, University of Zagreb, Zagreb 10000, Croatia. Electronic address: gruic@chem.pmf.hr.

Articles similaires

Bioinspired gelated cell sheet-supported lactobacillus biofilm for aerobic vaginitis diagnosis and treatment.

Yueyue Gui, Qingfei Sun, Kexin Li et al.
1.00
Female Biofilms Animals Lactobacillus Mice

Prediction of strain level phage-host interactions across the Escherichia genus using only genomic information.

Baptiste Gaborieau, Hugo Vaysset, Florian Tesson et al.
1.00
Host Specificity Bacteriophages Genomics Algorithms Escherichia coli

Equine bone marrow-derived mesenchymal stromal cells reduce established S. aureus and E. coli biofilm matrix in vitro.

Sarah M Khatibzadeh, Linda A Dahlgren, Clayton C Caswell et al.
1.00
Biofilms Horses Animals Escherichia coli Mesenchymal Stem Cells

Aminoacid functionalised magnetite nanoparticles Fe

Spoială Angela, Motelica Ludmila, Ilie Cornelia-Ioana et al.
1.00
Magnetite Nanoparticles Tryptophan Biocompatible Materials Microbial Sensitivity Tests Humans

Classifications MeSH

questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Enterobacteriaceae › Escherichia › Escherichia coli › On the Mechanism and Origin of Isoleucyl-tRNA...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Protéines Escherichia coli › On the Mechanism and Origin of Isoleucyl-tRNA...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Ligases › Carbon-oxygen ligases › Amino acyl-tRNA synthetases › Isoleucine-tRNA ligase › On the Mechanism and Origin of Isoleucyl-tRNA...
questionsmedicales.fr › Phénomènes génétiques › Expression des gènes › Biosynthèse des protéines › On the Mechanism and Origin of Isoleucyl-tRNA...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Maturation post-transcriptionnelle des ARN › Édition des ARN › On the Mechanism and Origin of Isoleucyl-tRNA...
questionsmedicales.fr › Acides aminés, peptides et protéines › Acides aminés › Acides aminés essentiels › Valine › On the Mechanism and Origin of Isoleucyl-tRNA...