A novel thermostable and efficient Class II glucose isomerase from the thermophilic Caldicoprobacter algeriensis: Biochemical characterization, molecular investigation, and application in High Fructose Syrup production.

Aldose-Ketose Isomerases / chemistry Amino Acid Sequence Bacteria / classification Chemical Phenomena Cloning, Molecular Enzyme Activation Enzyme Stability Fructose / metabolism Gene Expression High Fructose Corn Syrup / metabolism Hydrogen-Ion Concentration Kinetics Models, Molecular Molecular Conformation Phylogeny Recombinant Proteins Structure-Activity Relationship Temperature Thermodynamics

Caldicoprobacter algeriensis Catalytic efficiency Glucose isomerase High fructose syrup Molecular modeling Specific activity

Journal

International journal of biological macromolecules

ISSN: 1879-0003

Titre abrégé: Int J Biol Macromol

Pays: Netherlands

ID NLM: 7909578

Informations de publication

Date de publication:
15 May 2019

Historique:

received: 16 12 2018

revised: 18 01 2019

accepted: 26 01 2019

pubmed: 5 2 2019

medline: 23 7 2019

entrez: 5 2 2019

Statut: ppublish

Résumé

A novel glucose isomerase gene from the thermophilic Caldicoprobacter algeriensis, encoding a polypeptide of 438 residues, was identified, cloned and successfully expressed in E. coli. The purified enzyme (GICA) was a homotetramer of about 200 kDa displaying the highest activity at pH 7.0 and 90 °C and retaining 97% of its maximum activity at pH 6.5. The enzyme showed an excellent thermostability with a half-life of 6 min at 100 °C. Interestingly, GICA had a very high affinity of 40 mM and catalytic efficiency of 194 min

Identifiants

DOI: 10.1016/j.ijbiomac.2019.01.150 PMID: 30716378

pubmed: 30716378

pii: S0141-8130(18)36974-5

doi: 10.1016/j.ijbiomac.2019.01.150

pii:

doi:

Substances chimiques

High Fructose Corn Syrup 0

Recombinant Proteins 0

Fructose 30237-26-4

Aldose-Ketose Isomerases EC 5.3.1.-

xylose isomerase EC 5.3.1.5

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

Pagination

31-40

A novel thermostable and efficient Class II glucose isomerase from the thermophilic Caldicoprobacter algeriensis: Biochemical characterization, molecular investigation, and application in High Fructose Syrup production.

Journal

Informations de publication

Résumé

Identifiants

Substances chimiques

Types de publication

Langues

Sous-ensembles de citation

Pagination

Informations de copyright

Auteurs

Sawssan Neifar (S)

Hajer Ben Hlima (HB)

Sonia Mhiri (S)

Monia Mezghani (M)

Khelifa Bouacem (K)

Adel Hadj Ibrahim (AH)

Bassem Jaouadi (B)

Amel Bouanane-Darenfed (A)

Samir Bejar (S)

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Classifications MeSH