Blockage of the P2X7 Receptor Attenuates Harmful Changes Produced by Ischemia and Reperfusion in the Myenteric Plexus.
Animals
Cytoprotection
Disease Models, Animal
Gastrointestinal Motility
/ drug effects
Ileum
/ innervation
Male
Mesenteric Ischemia
/ drug therapy
Myenteric Plexus
/ drug effects
Neurons
/ drug effects
Neutrophil Infiltration
/ drug effects
Purinergic P2X Receptor Antagonists
/ pharmacology
Rats, Wistar
Receptors, Purinergic P2X7
/ drug effects
Reperfusion Injury
/ metabolism
Rosaniline Dyes
/ pharmacology
Signal Transduction
/ drug effects
Brilliant Blue G
Ileum
Ischemia and reperfusion
Myenteric plexus
P2X7 receptor
Journal
Digestive diseases and sciences
ISSN: 1573-2568
Titre abrégé: Dig Dis Sci
Pays: United States
ID NLM: 7902782
Informations de publication
Date de publication:
07 2019
07 2019
Historique:
received:
15
12
2017
accepted:
24
01
2019
pubmed:
9
2
2019
medline:
24
12
2019
entrez:
9
2
2019
Statut:
ppublish
Résumé
Our work analyzed the effects of a P2X7 receptor antagonist, Brilliant Blue G (BBG), on rat ileum myenteric plexus following ischemia and reperfusion (ISR) induced by 45 min of ileal artery occlusion with an atraumatic vascular clamp with 24 h (ISR 24-h group) or 14 d of reperfusion (ISR 14-d group). Either BBG (50 mg/kg or 100 mg/kg, BBG50 or BBG100 groups) or saline (vehicle) was administered subcutaneously 1 h after ischemia in the ISR 24-h group or once daily for the 5 d after ischemia in the ISR 14-d group (n = 5 per group). We evaluated the neuronal density and profile area by examining the number of neutrophils in the intestinal layers, protein expression levels of the P2X7 receptor, intestinal motility and immunoreactivity for the P2X7 receptor, nitric oxide synthase, neurofilament-200, and choline acetyl transferase in myenteric neurons. The neuronal density and profile area were restored by BBG following ISR. The ischemic groups showed alterations in P2X7 receptor protein expression and the number of neutrophils in the intestine and decreased intestinal motility, all of which were recovered by BBG treatment. We concluded that ISR morphologically and functionally affected the intestine and that its effects were reversed by BBG treatment, suggesting the P2X7 receptor as a therapeutic target.
Identifiants
pubmed: 30734238
doi: 10.1007/s10620-019-05496-8
pii: 10.1007/s10620-019-05496-8
doi:
Substances chimiques
P2rx7 protein, rat
0
Purinergic P2X Receptor Antagonists
0
Receptors, Purinergic P2X7
0
Rosaniline Dyes
0
coomassie Brilliant Blue
M1ZRX790SI
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1815-1829Références
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