OsSPL18 controls grain weight and grain number in rice.

Base Sequence Gene Expression Regulation, Plant / genetics Genetic Pleiotropy / genetics Oryza / genetics Plant Proteins / genetics Plants, Genetically Modified Transcription Factors / deficiency
DEP1 Grain number Grain weight OsSPL18 Rice

Journal

Journal of genetics and genomics = Yi chuan xue bao
ISSN: 1673-8527
Titre abrégé: J Genet Genomics
Pays: China
ID NLM: 101304616

Informations de publication

Date de publication:
20 01 2019
Historique:
received: 07 11 2018
revised: 24 12 2018
accepted: 20 01 2019
pubmed: 10 2 2019
medline: 24 3 2020
entrez: 10 2 2019
Statut: ppublish

Résumé

Grain weight and grain number are two important traits directly determining grain yield in rice. To date, a lot of genes related to grain weight and grain number have been identified; however, the regulatory mechanism underlying these genes remains largely unknown. In this study, we studied the biological function of OsSPL18 during grain and panicle development in rice. Knockout (KO) mutants of OsSPL18 exhibited reduced grain width and thickness, panicle length and grain number, but increased tiller number. Cytological analysis showed that OsSPL18 regulates the development of spikelet hulls by affecting cell proliferation. qRT-PCR and GUS staining analyses showed that OsSPL18 was highly expressed in developing young panicles and young spikelet hulls, in agreement with its function in regulating grain and panicle development. Transcriptional activation experiments indicated that OsSPL18 is a functional transcription factor with activation domains in both the N-terminus and C-terminus, and both activation domains are indispensable for its biological functions. Quantitative expression analysis showed that DEP1, a major grain number regulator, was significantly down-regulated in OsSPL18 KO lines. Both yeast one-hybrid and dual-luciferase (LUC) assays showed that OsSPL18 could bind to the DEP1 promoter, suggesting that OsSPL18 regulates panicle development by positively regulating the expression of DEP1. Sequence analysis showed that OsSPL18 contains the OsmiR156k complementary sequence in the third exon; 5' RLM-RACE experiments indicated that OsSPL18 could be cleaved by OsmiR156k. Taken together, our results uncovered a new OsmiR156k-OsSPL18-DEP1 pathway regulating grain number in rice.

Identifiants

DOI: 10.1016/j.jgg.2019.01.003 PMID: 30737149
pubmed: 30737149
pii: S1673-8527(19)30014-1
doi: 10.1016/j.jgg.2019.01.003
pii:
doi:

Substances chimiques

Plant Proteins 0
Transcription Factors 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

41-51

Informations de copyright

Copyright © 2019 The Authors. Published by Elsevier Ltd.. All rights reserved.

Auteurs

Hua Yuan (H)

Rice Research Institute, Sichuan Agricultural University, Wenjiang, 611130, China; State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Wenjiang, 611130, China.

Peng Qin (P)

Rice Research Institute, Sichuan Agricultural University, Wenjiang, 611130, China; State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Wenjiang, 611130, China.

Li Hu (L)

Rice Research Institute, Sichuan Agricultural University, Wenjiang, 611130, China; State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Wenjiang, 611130, China.

Shijie Zhan (S)

Rice Research Institute, Sichuan Agricultural University, Wenjiang, 611130, China; State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Wenjiang, 611130, China.

Shifu Wang (S)

Rice Research Institute, Sichuan Agricultural University, Wenjiang, 611130, China; State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Wenjiang, 611130, China.

Peng Gao (P)

Rice Research Institute, Sichuan Agricultural University, Wenjiang, 611130, China; State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Wenjiang, 611130, China.

Jing Li (J)

Rice Research Institute, Sichuan Agricultural University, Wenjiang, 611130, China; Station of Foundation and Improved Seed-breeding of Sichuan Province, Shuangliu, 610200, China.

Mengya Jin (M)

Rice Research Institute, Sichuan Agricultural University, Wenjiang, 611130, China; State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Wenjiang, 611130, China.

Zhengyan Xu (Z)

Rice Research Institute, Sichuan Agricultural University, Wenjiang, 611130, China; State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Wenjiang, 611130, China.

Qiang Gao (Q)

State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.

Anping Du (A)

Rice Research Institute, Sichuan Agricultural University, Wenjiang, 611130, China.

Bin Tu (B)

Rice Research Institute, Sichuan Agricultural University, Wenjiang, 611130, China; State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Wenjiang, 611130, China.

Weilan Chen (W)

Rice Research Institute, Sichuan Agricultural University, Wenjiang, 611130, China; State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Wenjiang, 611130, China.

Bingtian Ma (B)

Rice Research Institute, Sichuan Agricultural University, Wenjiang, 611130, China; State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Wenjiang, 611130, China.

Yuping Wang (Y)

Rice Research Institute, Sichuan Agricultural University, Wenjiang, 611130, China; State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Wenjiang, 611130, China.

Shigui Li (S)

Rice Research Institute, Sichuan Agricultural University, Wenjiang, 611130, China; State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Wenjiang, 611130, China. Electronic address: lishigui@sicau.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Sciences de l'information › Sources d'information › Services d'information › Documentation › Données de séquences moléculaires › Séquence nucléotidique › OsSPL18 controls grain weight and grain number in rice.
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Régulation de l'expression des gènes végétaux › OsSPL18 controls grain weight and grain number in rice.
questionsmedicales.fr › Phénomènes génétiques › Phénotype › Pléiotropie › OsSPL18 controls grain weight and grain number in rice.
questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Poaceae › Oryza › OsSPL18 controls grain weight and grain number in rice.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines végétales › OsSPL18 controls grain weight and grain number in rice.
questionsmedicales.fr › Stades d'organismes › Organismes génétiquement modifiés › Végétaux génétiquement modifiés › OsSPL18 controls grain weight and grain number in rice.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › OsSPL18 controls grain weight and grain number in rice.