Activation of the Endonuclease that Defines mRNA 3' Ends Requires Incorporation into an 8-Subunit Core Cleavage and Polyadenylation Factor Complex.

Cryoelectron Microscopy Crystallography, X-Ray Cytochromes c / genetics DNA-Binding Proteins / genetics Endonucleases / genetics Enzyme Activation Mitochondrial Proteins / genetics Molecular Docking Simulation Multiprotein Complexes Polyadenylation Polynucleotide Adenylyltransferase / genetics Protein Binding Protein Interaction Domains and Motifs RNA Precursors / genetics RNA, Fungal / genetics RNA, Messenger / genetics Saccharomyces cerevisiae / enzymology Saccharomyces cerevisiae Proteins / genetics Spectrometry, Mass, Electrospray Ionization Structure-Activity Relationship Tandem Mass Spectrometry mRNA Cleavage and Polyadenylation Factors / genetics
X-ray crystallography baculovirus cleavage cryo-EM hydrogen-deuterium exchange mRNA mass spectrometry nuclease polyadenylation pre-mRNA

Journal

Molecular cell
ISSN: 1097-4164
Titre abrégé: Mol Cell
Pays: United States
ID NLM: 9802571

Informations de publication

Date de publication:
21 03 2019
Historique:
received: 13 09 2018
revised: 02 11 2018
accepted: 21 12 2018
pubmed: 10 2 2019
medline: 15 9 2020
entrez: 10 2 2019
Statut: ppublish

Résumé

Cleavage and polyadenylation factor (CPF/CPSF) is a multi-protein complex essential for formation of eukaryotic mRNA 3' ends. CPF cleaves pre-mRNAs at a specific site and adds a poly(A) tail. The cleavage reaction defines the 3' end of the mature mRNA, and thus the activity of the endonuclease is highly regulated. Here, we show that reconstitution of specific pre-mRNA cleavage with recombinant yeast proteins requires incorporation of the Ysh1 endonuclease into an eight-subunit "CPF

Identifiants

DOI: 10.1016/j.molcel.2018.12.023 PMID: 30737185 PMC: PMC6436931
pubmed: 30737185
pii: S1097-2765(18)31099-2
doi: 10.1016/j.molcel.2018.12.023
pmc: PMC6436931
pii:
doi:

Substances chimiques

CFT2 protein, S cerevisiae 0
CYC1 protein, S cerevisiae 0
DNA-Binding Proteins 0
MGM101 protein, S cerevisiae 0
Mitochondrial Proteins 0
Mpe1 protein, S cerevisiae 0
Multiprotein Complexes 0
RNA Precursors 0
RNA, Fungal 0
RNA, Messenger 0
Saccharomyces cerevisiae Proteins 0
YSH1 protein, S cerevisiae 0
mRNA Cleavage and Polyadenylation Factors 0
Cytochromes c 9007-43-6
PAP1 protein, S cerevisiae EC 2.7.7.19
Polynucleotide Adenylyltransferase EC 2.7.7.19
Endonucleases EC 3.1.-

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1217-1231.e11

Subventions

Organisme : Medical Research Council
ID : MC_U105184326
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_U105192715
Pays : United Kingdom
Organisme : European Research Council
ID : 725685
Pays : International
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : European Research Council
ID : 261151
Pays : International

Informations de copyright

Copyright © 2019 MRC Laboratory of Molecular Biology. Published by Elsevier Inc. All rights reserved.

Références

Cell. 1981 Apr;24(1):251-60
pubmed: 6113054
Nat Struct Mol Biol. 2018 Feb;25(2):135-138
pubmed: 29358758
Anal Chem. 2005 Apr 1;77(7):2187-200
pubmed: 15801753
J Struct Biol. 2005 Oct;152(1):36-51
pubmed: 16182563
Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):213-21
pubmed: 20124702
Mol Cell Biol. 1985 Feb;5(2):373-9
pubmed: 2579321
Nature. 2017 May 25;545(7655):505-509
pubmed: 28514442
Science. 1996 Nov 29;274(5292):1511-4
pubmed: 8929408
Nucleic Acids Res. 2017 Sep 29;45(17):10115-10131
pubmed: 28973460
Structure. 2011 Apr 13;19(4):534-45
pubmed: 21481776
Genes Dev. 2015 May 1;29(9):889-97
pubmed: 25934501
Biochemistry. 2008 Jul 1;47(26):6859-69
pubmed: 18537269
Science. 2012 May 25;336(6084):1030-3
pubmed: 22628654
Nucleic Acids Res. 2007;35(1):87-99
pubmed: 17151076
Genes Dev. 2014 Oct 15;28(20):2248-60
pubmed: 25319826
Genes Dev. 2011 Sep 1;25(17):1770-82
pubmed: 21896654
J Pharm Sci. 2011 Jun;100(6):2071-86
pubmed: 21491437
Nature. 2006 Dec 14;444(7121):953-6
pubmed: 17128255
Proc Natl Acad Sci U S A. 2001 May 22;98(11):6080-5
pubmed: 11344258
Proc Natl Acad Sci U S A. 2018 Feb 13;115(7):E1419-E1428
pubmed: 29208711
J Struct Biol. 2007 Jan;157(1):38-46
pubmed: 16859925
Science. 2017 Nov 24;358(6366):1056-1059
pubmed: 29074584
EMBO J. 1998 Dec 15;17(24):7454-68
pubmed: 9857200
J Am Soc Mass Spectrom. 2012 Jan;23(1):76-87
pubmed: 22038510
J Comput Chem. 2004 Oct;25(13):1605-12
pubmed: 15264254
Mol Cell Biol. 1999 Nov;19(11):7733-40
pubmed: 10523662
Nucleic Acids Res. 2010 May;38(9):3119-32
pubmed: 20097654
J Struct Biol. 2016 Jan;193(1):1-12
pubmed: 26592709
RNA. 2017 Jan;23(1):98-107
pubmed: 27780845
Biochimie. 2014 Jun;101:203-7
pubmed: 24508575
EMBO J. 2000 Jul 17;19(14):3778-87
pubmed: 10899131
Acta Crystallogr D Biol Crystallogr. 2010 Jan;66(Pt 1):12-21
pubmed: 20057044
Nucleic Acids Res. 2004 Jul 1;32(Web Server issue):W665-7
pubmed: 15215472
Genes Dev. 2005 Nov 1;19(21):2583-92
pubmed: 16230528
Nature. 2004 Jul 8;430(6996):223-6
pubmed: 15241417
Mol Cell Biol. 2014 Jun;34(11):1894-910
pubmed: 24591651
Genes Dev. 2014 Nov 1;28(21):2381-93
pubmed: 25301781
Science. 2014 Dec 12;346(6215):1377-80
pubmed: 25504723
Biochemistry. 2011 Nov 29;50(47):10203-14
pubmed: 22026644
Acta Crystallogr D Biol Crystallogr. 2008 Jan;64(Pt 1):61-9
pubmed: 18094468
J Am Soc Mass Spectrom. 2015 Jan;26(1):83-97
pubmed: 25261217
Genes Dev. 1997 Oct 1;11(19):2545-56
pubmed: 9334319
RNA. 1999 Feb;5(2):272-80
pubmed: 10024178
Nat Struct Mol Biol. 2014 Feb;21(2):175-179
pubmed: 24413056
Mol Cell Biol. 2001 Mar;21(6):2026-37
pubmed: 11238938
Cell Mol Life Sci. 2008 Apr;65(7-8):1099-122
pubmed: 18158581
Proc Natl Acad Sci U S A. 2016 May 10;113(19):E2564-9
pubmed: 27114506
Mol Cell. 2014 Sep 4;55(5):745-57
pubmed: 25192364
J Mol Biol. 2007 Sep 21;372(3):774-97
pubmed: 17681537
J Mol Biol. 2016 Feb 22;428(4):720-725
pubmed: 26410586
Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):486-501
pubmed: 20383002
Mol Cell Biol. 2014 Nov;34(21):3955-67
pubmed: 25135474
Science. 2016 Sep 23;353(6306):
pubmed: 27708008
Proc Natl Acad Sci U S A. 2001 Aug 28;98(18):10037-41
pubmed: 11517324
J Biol Chem. 2007 Jan 19;282(3):2101-15
pubmed: 17116658
J Biol Chem. 2014 Dec 5;289(49):33916-26
pubmed: 25339178
RNA. 2004 Apr;10(4):565-73
pubmed: 15037765
Mol Cell Biol. 2005 Feb;25(4):1489-500
pubmed: 15684398
J Biol Chem. 2003 Aug 29;278(35):33000-10
pubmed: 12819204
Elife. 2017 Dec 23;6:
pubmed: 29274231
Science. 1988 Dec 2;242(4883):1270-4
pubmed: 2848317
Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):125-32
pubmed: 20124692
Nucleic Acids Res. 2003 Mar 15;31(6):1744-52
pubmed: 12626716
Acta Crystallogr D Biol Crystallogr. 2013 Jul;69(Pt 7):1204-14
pubmed: 23793146
Nature. 2004 Nov 25;432(7016):517-22
pubmed: 15565157
Mol Cell Biol. 1996 Jun;16(6):2772-6
pubmed: 8649385
J Mol Biol. 2010 Aug 20;401(3):334-49
pubmed: 20600122
Nat Struct Mol Biol. 2017 Oct 5;24(10):791-799
pubmed: 28981077
Proc Natl Acad Sci U S A. 1994 Jan 4;91(1):257-61
pubmed: 8278376
Mol Cell. 2009 Feb 13;33(3):365-76
pubmed: 19217410
Wiley Interdiscip Rev RNA. 2010 Sep-Oct;1(2):325-40
pubmed: 21935893
PLoS One. 2011;6(12):e29139
pubmed: 22216186
EMBO J. 2001 Jun 15;20(12):3197-209
pubmed: 11406596
Wiley Interdiscip Rev RNA. 2012 May-Jun;3(3):385-96
pubmed: 22012871
RNA. 2012 Jun;18(6):1154-62
pubmed: 22513198
Nat Methods. 2017 Apr;14(4):331-332
pubmed: 28250466
J Appl Crystallogr. 2007 Aug 1;40(Pt 4):658-674
pubmed: 19461840
EMBO J. 2006 Jul 12;25(13):3167-78
pubmed: 16794580
Anal Chem. 2010 Aug 15;82(16):6958-68
pubmed: 20704385
Science. 1996 Nov 29;274(5292):1514-7
pubmed: 8929409
Steroids. 2000 Jun;65(6):295-303
pubmed: 10802280
Curr Protoc Protein Sci. 2008 Feb;Chapter 5:Unit 5.20
pubmed: 18429060
Bioinformatics. 2008 Nov 1;24(21):2534-6
pubmed: 18606607
Nat Commun. 2017 Sep 20;8(1):629
pubmed: 28931821
J Struct Biol. 2012 Dec;180(3):519-30
pubmed: 23000701
Proteins. 2005 Dec 1;61(4):704-21
pubmed: 16231289
BMC Struct Biol. 2006 Jan 05;6:1
pubmed: 16396680

Auteurs

Chris H Hill (CH)

MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK.

Vytautė Boreikaitė (V)

MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK.

Ananthanarayanan Kumar (A)

MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK.

Ana Casañal (A)

MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK.

Peter Kubík (P)

MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK.

Gianluca Degliesposti (G)

MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK.

Sarah Maslen (S)

MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK.

Angelica Mariani (A)

MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK.

Ottilie von Loeffelholz (O)

Centre for Integrative Biology, Department of Integrated Structural Biology, Institute of Genetics and of Molecular and Cellular Biology, Illkirch, Université de Strasbourg, Strasbourg, France; Centre National de la Recherche Scientifique UMR 7104, Illkirch, Université de Strasbourg, Strasbourg, France; INSERM U964, Illkirch, Université de Strasbourg, Strasbourg, France.

Mathias Girbig (M)

MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK.

Mark Skehel (M)

MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK.

Lori A Passmore (LA)

MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK. Electronic address: passmore@mrc-lmb.cam.ac.uk.

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Microscopie électronique › Cryomicroscopie électronique › Activation of the Endonuclease that Defines...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Cristallographie aux rayons X › Activation of the Endonuclease that Defines...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Hémoprotéines › Cytochromes › Cytochromes de type c › Cytochromes c › Activation of the Endonuclease that Defines...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines de liaison à l'ADN › Activation of the Endonuclease that Defines...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Esterases › Endonucleases › Activation of the Endonuclease that Defines...
questionsmedicales.fr › Métabolisme › Activation enzymatique › Activation of the Endonuclease that Defines...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines mitochondriales › Activation of the Endonuclease that Defines...
questionsmedicales.fr › Sciences de l'information › Méthodologies informatiques › Simulation numérique › Simulation de docking moléculaire › Activation of the Endonuclease that Defines...
questionsmedicales.fr › Structures macromoléculaires › Complexes multiprotéiques › Activation of the Endonuclease that Defines...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Maturation post-transcriptionnelle des ARN › Maturation de l'extrémité 3' des ARN › Polyadénylation › Activation of the Endonuclease that Defines...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Transferases › Phosphotransferases › Nucleotidyltransferases › Polynucleotide adenylyltransferase › Activation of the Endonuclease that Defines...
questionsmedicales.fr › Métabolisme › Liaison aux protéines › Activation of the Endonuclease that Defines...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Éléments structuraux des protéines › Motifs et domaines d'intéraction protéique › Activation of the Endonuclease that Defines...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › Précurseurs des ARN › Activation of the Endonuclease that Defines...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN fongique › Activation of the Endonuclease that Defines...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN messager › Activation of the Endonuclease that Defines...
questionsmedicales.fr › Eucaryotes › Champignons › Levures › Saccharomyces › Saccharomyces cerevisiae › Activation of the Endonuclease that Defines...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines fongiques › Protéines de Saccharomyces cerevisiae › Activation of the Endonuclease that Defines...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Spectrométrie de masse ESI › Activation of the Endonuclease that Defines...
questionsmedicales.fr › Phénomènes physiologiques › Phénomènes pharmacologiques et toxicologiques › Phénomènes pharmacologiques › Relation structure-activité › Activation of the Endonuclease that Defines...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Spectrométrie de masse en tandem › Activation of the Endonuclease that Defines...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Nucléoprotéines › Protéines de liaison à l'ARN › Facteurs de clivage et de polyadénylation de l'ARN messager › Activation of the Endonuclease that Defines...