Diagnosis and characterization of canine distemper virus through sequencing by MinION nanopore technology.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
08 02 2019
08 02 2019
Historique:
received:
18
06
2018
accepted:
06
12
2018
entrez:
10
2
2019
pubmed:
10
2
2019
medline:
26
8
2020
Statut:
epublish
Résumé
Prompt identification of the causative pathogen of an infectious disease is essential for the choice of treatment or preventive measures. In this perspective, nucleic acids purified from the brain tissue of a dog succumbed after severe neurological signs were processed with the MinION (Oxford Nanopore Technologies, Oxford UK) sequencing technology. Canine distemper virus (CDV) sequence reads were detected. Subsequently, a specific molecular test and immunohistochemistry were used to confirm the presence of CDV RNA and antigen, respectively, in tissues. This study supports the use of the NGS in veterinary clinical practice with potential advantages in terms of rapidity and broad-range of molecular diagnosis.
Identifiants
pubmed: 30737428
doi: 10.1038/s41598-018-37497-4
pii: 10.1038/s41598-018-37497-4
pmc: PMC6368598
doi:
Substances chimiques
Antigens, Viral
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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