Concerted expression of a cell cycle regulator and a metabolic enzyme from a bicistronic transcript in plants.
5' Untranslated Regions
Acid Anhydride Hydrolases
/ genetics
Anaphase-Promoting Complex-Cyclosome
/ genetics
Arabidopsis
/ cytology
Arabidopsis Proteins
/ genetics
CRISPR-Cas Systems
Cytoplasm
/ metabolism
Gene Expression Regulation, Plant
Mutation
Open Reading Frames
Plants, Genetically Modified
Polyribosomes
/ genetics
Journal
Nature plants
ISSN: 2055-0278
Titre abrégé: Nat Plants
Pays: England
ID NLM: 101651677
Informations de publication
Date de publication:
02 2019
02 2019
Historique:
received:
10
08
2018
accepted:
04
01
2019
entrez:
10
2
2019
pubmed:
10
2
2019
medline:
2
7
2019
Statut:
ppublish
Résumé
Eukaryotic mRNAs frequently contain upstream open reading frames (uORFs), encoding small peptides that may control translation of the main ORF (mORF). Here, we report the characterization of a distinct bicistronic transcript in Arabidopsis. We analysed loss-of-function phenotypes of the inorganic polyphosphatase TRIPHOSPHATE TUNNEL METALLOENZYME 3 (AtTTM3), and found that catalytically inactive versions of the enzyme could fully complement embryo and growth-related phenotypes. We could rationalize these puzzling findings by characterizing a uORF in the AtTTM3 locus encoding CELL DIVISION CYCLE PROTEIN 26 (CDC26), an orthologue of the cell cycle regulator. We demonstrate that AtCDC26 is part of the plant anaphase promoting complex/cyclosome (APC/C), regulates accumulation of APC/C target proteins and controls cell division, growth and embryo development. AtCDC26 and AtTTM3 are translated from a single transcript conserved across the plant lineage. While there is no apparent biochemical connection between the two gene products, AtTTM3 coordinates AtCDC26 translation by recruiting the transcript into polysomes. Our work highlights that uORFs may encode functional proteins in plant genomes.
Identifiants
pubmed: 30737513
doi: 10.1038/s41477-019-0358-3
pii: 10.1038/s41477-019-0358-3
doi:
Substances chimiques
5' Untranslated Regions
0
Arabidopsis Proteins
0
Anaphase-Promoting Complex-Cyclosome
EC 2.3.2.27
Acid Anhydride Hydrolases
EC 3.6.-
At2g11890 protein, Arabidopsis
EC 3.6.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
184-193Références
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