Targeted expression and purification of fluorine labelled cold shock protein B by using an auxotrophic strategy.
Bacillus subtilis
/ chemistry
Bacterial Proteins
/ chemistry
Escherichia coli
/ genetics
Fluorine
/ chemistry
Gene Expression
Halogenation
Models, Molecular
Nuclear Magnetic Resonance, Biomolecular
/ methods
Phenylalanine
/ analogs & derivatives
Spectrometry, Mass, Electrospray Ionization
/ methods
Tryptophan
/ analogs & derivatives
(19)F labelling
Auxotrophy
Cold shock protein B
E. coli
NMR spectroscopy
Recombinant protein expression
Journal
Protein expression and purification
ISSN: 1096-0279
Titre abrégé: Protein Expr Purif
Pays: United States
ID NLM: 9101496
Informations de publication
Date de publication:
05 2019
05 2019
Historique:
received:
19
12
2018
revised:
05
02
2019
accepted:
05
02
2019
pubmed:
10
2
2019
medline:
10
4
2020
entrez:
10
2
2019
Statut:
ppublish
Résumé
High resolution NMR spectroscopy is a seminal method in modern structural biology to obtain insights into proteins' structure, dynamics and function at dilute condition as well as in a cell-like environment or even intracellularly. Usually,
Identifiants
pubmed: 30738179
pii: S1046-5928(18)30651-X
doi: 10.1016/j.pep.2019.02.006
pii:
doi:
Substances chimiques
Bacterial Proteins
0
cold-shock protein CspB, Bacteria
0
Fluorine
284SYP0193
Phenylalanine
47E5O17Y3R
Tryptophan
8DUH1N11BX
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
86-91Informations de copyright
Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.