Feasibility and utility of a panel testing for 114 cancer-associated genes in a clinical setting: A hospital-based study.


Journal

Cancer science
ISSN: 1349-7006
Titre abrégé: Cancer Sci
Pays: England
ID NLM: 101168776

Informations de publication

Date de publication:
Apr 2019
Historique:
received: 24 09 2018
revised: 21 01 2019
accepted: 06 02 2019
pubmed: 12 2 2019
medline: 17 4 2019
entrez: 12 2 2019
Statut: ppublish

Résumé

Next-generation sequencing (NGS) of tumor tissue (ie, clinical sequencing) can guide clinical management by providing information about actionable gene aberrations that have diagnostic and therapeutic significance. Here, we undertook a hospital-based prospective study (TOP-GEAR project, 2nd stage) to investigate the feasibility and utility of NGS-based analysis of 114 cancer-associated genes (the NCC Oncopanel test). We examined 230 cases (comprising more than 30 tumor types) of advanced solid tumors, all of which were matched with nontumor samples. Gene profiling data were obtained for 187 cases (81.3%), 111 (59.4%) of which harbored actionable gene aberrations according to the Clinical Practice Guidelines for Next Generation Sequencing in Cancer Diagnosis and Treatment (Edition 1.0) issued by 3 major Japanese cancer-related societies. Twenty-five (13.3%) cases have since received molecular-targeted therapy according to their gene aberrations. These results indicate the utility of tumor-profiling multiplex gene panel testing in a clinical setting in Japan. This study is registered with UMIN Clinical Trials Registry (UMIN 000011141).

Identifiants

pubmed: 30742731
doi: 10.1111/cas.13969
pmc: PMC6447843
doi:

Substances chimiques

Biomarkers, Tumor 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

1480-1490

Subventions

Organisme : National Cancer Center Research and Development Fund
ID : 27-A-1, 30-A-6
Organisme : Japan Agency for Medical Research and Development
ID : JP18kk0205004, JP18lk1403003

Informations de copyright

© 2019 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

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Auteurs

Kuniko Sunami (K)

Department of Pathology and Clinical Laboratories, National Cancer Center Hospital, Tokyo, Japan.

Hitoshi Ichikawa (H)

Department of Clinical Genomics, National Cancer Center Research Institute, Tokyo, Japan.
Division of Translational Genomics, Exploratory Oncology Research and Clinical Trial Center, National Cancer Center, Tokyo, Japan.

Takashi Kubo (T)

Department of Pathology and Clinical Laboratories, National Cancer Center Hospital, Tokyo, Japan.
Department of Clinical Genomics, National Cancer Center Research Institute, Tokyo, Japan.
Division of Translational Genomics, Exploratory Oncology Research and Clinical Trial Center, National Cancer Center, Tokyo, Japan.

Mamoru Kato (M)

Department of Bioinformatics, National Cancer Center Research Institute, Tokyo, Japan.

Yutaka Fujiwara (Y)

Department of Experimental Therapeutics, National Cancer Center Hospital, Tokyo, Japan.

Akihiko Shimomura (A)

Department of Experimental Therapeutics, National Cancer Center Hospital, Tokyo, Japan.

Takafumi Koyama (T)

Department of Experimental Therapeutics, National Cancer Center Hospital, Tokyo, Japan.

Hiroki Kakishima (H)

Department of Pathology and Clinical Laboratories, National Cancer Center Hospital, Tokyo, Japan.

Mayuko Kitami (M)

Department of Pathology and Clinical Laboratories, National Cancer Center Hospital, Tokyo, Japan.

Hiromichi Matsushita (H)

Department of Pathology and Clinical Laboratories, National Cancer Center Hospital, Tokyo, Japan.

Eisaku Furukawa (E)

Department of Bioinformatics, National Cancer Center Research Institute, Tokyo, Japan.

Daichi Narushima (D)

Department of Bioinformatics, National Cancer Center Research Institute, Tokyo, Japan.

Momoko Nagai (M)

Department of Bioinformatics, National Cancer Center Research Institute, Tokyo, Japan.

Hirokazu Taniguchi (H)

Department of Pathology and Clinical Laboratories, National Cancer Center Hospital, Tokyo, Japan.

Noriko Motoi (N)

Department of Pathology and Clinical Laboratories, National Cancer Center Hospital, Tokyo, Japan.

Shigeki Sekine (S)

Department of Pathology and Clinical Laboratories, National Cancer Center Hospital, Tokyo, Japan.

Akiko Maeshima (A)

Department of Pathology and Clinical Laboratories, National Cancer Center Hospital, Tokyo, Japan.

Taisuke Mori (T)

Department of Pathology and Clinical Laboratories, National Cancer Center Hospital, Tokyo, Japan.

Reiko Watanabe (R)

Department of Pathology and Clinical Laboratories, National Cancer Center Hospital, Tokyo, Japan.

Masayuki Yoshida (M)

Department of Pathology and Clinical Laboratories, National Cancer Center Hospital, Tokyo, Japan.

Akihiko Yoshida (A)

Department of Pathology and Clinical Laboratories, National Cancer Center Hospital, Tokyo, Japan.

Hiroshi Yoshida (H)

Department of Pathology and Clinical Laboratories, National Cancer Center Hospital, Tokyo, Japan.

Kaishi Satomi (K)

Department of Pathology and Clinical Laboratories, National Cancer Center Hospital, Tokyo, Japan.

Aoi Sukeda (A)

Department of Pathology and Clinical Laboratories, National Cancer Center Hospital, Tokyo, Japan.

Taiki Hashimoto (T)

Department of Pathology and Clinical Laboratories, National Cancer Center Hospital, Tokyo, Japan.

Toshio Shimizu (T)

Department of Experimental Therapeutics, National Cancer Center Hospital, Tokyo, Japan.

Satoru Iwasa (S)

Department of Experimental Therapeutics, National Cancer Center Hospital, Tokyo, Japan.

Kan Yonemori (K)

Department of Experimental Therapeutics, National Cancer Center Hospital, Tokyo, Japan.

Ken Kato (K)

Department of Gastrointestinal Medical Oncology, National Cancer Center Hospital, Tokyo, Japan.

Chigusa Morizane (C)

Department of Hepatobiliary and Pancreatic Oncology, National Cancer Center Hospital, Tokyo, Japan.

Chitose Ogawa (C)

Department of Pediatric Oncology, National Cancer Center Hospital, Tokyo, Japan.

Noriko Tanabe (N)

Genetic Medicine and Services, National Cancer Center Hospital, Tokyo, Japan.

Kokichi Sugano (K)

Genetic Medicine and Services, National Cancer Center Hospital, Tokyo, Japan.
Oncogene Research Unit/Cancer Prevention Unit, Tochigi Cancer Center Research Institute, Tochigi, Japan.

Nobuyoshi Hiraoka (N)

Department of Pathology and Clinical Laboratories, National Cancer Center Hospital, Tokyo, Japan.

Kenji Tamura (K)

Department of Breast and Medical Oncology, National Cancer Center Hospital, Tokyo, Japan.

Teruhiko Yoshida (T)

Genetic Medicine and Services, National Cancer Center Hospital, Tokyo, Japan.

Yasuhiro Fujiwara (Y)

Department of Breast and Medical Oncology, National Cancer Center Hospital, Tokyo, Japan.
Strategic Planning Bureau, National Cancer Center, Tokyo, Japan.

Atsushi Ochiai (A)

Exploratory Oncology Research and Clinical Trial Center, National Cancer Center, Tokyo, Japan.

Noboru Yamamoto (N)

Department of Experimental Therapeutics, National Cancer Center Hospital, Tokyo, Japan.

Takashi Kohno (T)

Division of Translational Genomics, Exploratory Oncology Research and Clinical Trial Center, National Cancer Center, Tokyo, Japan.
Division of Genome Biology, National Cancer Center Research Institute, Tokyo, Japan.

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