Gut microbiome patterns correlate with higher postoperative complication rates after pancreatic surgery.
Aged
Bacteria
/ classification
Feces
/ microbiology
Gastrointestinal Microbiome
High-Throughput Nucleotide Sequencing
Humans
Middle Aged
Odds Ratio
Pancreatic Diseases
/ surgery
Phylogeny
Pilot Projects
Postoperative Complications
/ microbiology
Prospective Studies
RNA, Ribosomal, 16S
/ genetics
Risk Factors
16S RNA gene sequencing
Gut microbiome
Inflammation
Pancreas
Postoperative complications
Sepsis
Journal
BMC microbiology
ISSN: 1471-2180
Titre abrégé: BMC Microbiol
Pays: England
ID NLM: 100966981
Informations de publication
Date de publication:
18 02 2019
18 02 2019
Historique:
received:
05
07
2018
accepted:
23
01
2019
entrez:
20
2
2019
pubmed:
20
2
2019
medline:
18
12
2019
Statut:
epublish
Résumé
Postoperative complications are of great relevance in daily clinical practice, and the gut microbiome might play an important role by preventing pathogens from crossing the intestinal barrier. The two aims of this prospective clinical pilot study were: (1) to examine changes in the gut microbiome following pancreatic surgery, and (2) to correlate these changes with the postoperative course of the patient. In total, 116 stool samples of 32 patients undergoing pancreatic surgery were analysed by 16S-rRNA gene next-generation sequencing. One sample per patient was collected preoperatively in order to determine the baseline gut microbiome without exposure to surgical stress and/or antibiotic use. At least two further samples were obtained within the first 10 days following the surgical procedure to observe longitudinal changes in the gut microbiome. Whenever complications occurred, further samples were examined. Based on the structure of the gut microbiome, the samples could be allocated into three different microbial communities (A, B and C). Community B showed an increase in Akkermansia, Enterobacteriaceae and Bacteroidales as well as a decrease in Lachnospiraceae, Prevotella and Bacteroides. Patients showing a microbial composition resembling community B at least once during the observation period were found to have a significantly higher risk for developing postoperative complications (B vs. A, odds ratio = 4.96, p < 0.01**; B vs. C, odds ratio = 2.89, p = 0.019*). The structure of the gut microbiome is associated with the development of postoperative complications.
Sections du résumé
BACKGROUND
Postoperative complications are of great relevance in daily clinical practice, and the gut microbiome might play an important role by preventing pathogens from crossing the intestinal barrier. The two aims of this prospective clinical pilot study were: (1) to examine changes in the gut microbiome following pancreatic surgery, and (2) to correlate these changes with the postoperative course of the patient.
RESULTS
In total, 116 stool samples of 32 patients undergoing pancreatic surgery were analysed by 16S-rRNA gene next-generation sequencing. One sample per patient was collected preoperatively in order to determine the baseline gut microbiome without exposure to surgical stress and/or antibiotic use. At least two further samples were obtained within the first 10 days following the surgical procedure to observe longitudinal changes in the gut microbiome. Whenever complications occurred, further samples were examined. Based on the structure of the gut microbiome, the samples could be allocated into three different microbial communities (A, B and C). Community B showed an increase in Akkermansia, Enterobacteriaceae and Bacteroidales as well as a decrease in Lachnospiraceae, Prevotella and Bacteroides. Patients showing a microbial composition resembling community B at least once during the observation period were found to have a significantly higher risk for developing postoperative complications (B vs. A, odds ratio = 4.96, p < 0.01**; B vs. C, odds ratio = 2.89, p = 0.019*).
CONCLUSIONS
The structure of the gut microbiome is associated with the development of postoperative complications.
Identifiants
pubmed: 30777006
doi: 10.1186/s12866-019-1399-5
pii: 10.1186/s12866-019-1399-5
pmc: PMC6379976
doi:
Substances chimiques
RNA, Ribosomal, 16S
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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