Microbial residence time is a controlling parameter of the taxonomic composition and functional profile of microbial communities.
Journal
The ISME journal
ISSN: 1751-7370
Titre abrégé: ISME J
Pays: England
ID NLM: 101301086
Informations de publication
Date de publication:
06 2019
06 2019
Historique:
received:
07
08
2018
accepted:
01
02
2019
revised:
29
01
2019
pubmed:
23
2
2019
medline:
23
11
2019
entrez:
22
2
2019
Statut:
ppublish
Résumé
A remaining challenge within microbial ecology is to understand the determinants of richness and diversity observed in environmental microbial communities. In a range of systems, including activated sludge bioreactors, the microbial residence time (MRT) has been previously shown to shape the microbial community composition. However, the physiological and ecological mechanisms driving this influence have remained unclear. Here, this relationship is explored by analyzing an activated sludge system fed with municipal wastewater. Using a model designed in this study based on Monod-growth kinetics, longer MRTs were shown to increase the range of growth parameters that enable persistence, resulting in increased richness and diversity in the modeled community. In laboratory experiments, six sequencing batch reactors treating domestic wastewater were operated in parallel at MRTs between 1 and 15 days. The communities were characterized using both 16S ribosomal RNA and non-target messenger RNA sequencing (metatranscriptomic analysis), and model-predicted monotonic increases in richness were confirmed in both profiles. Accordingly, taxonomic Shannon diversity also increased with MRT. In contrast, the diversity in enzyme class annotations resulting from the metatranscriptomic analysis displayed a non-monotonic trend over the MRT gradient. Disproportionately high abundances of transcripts encoding for rarer enzymes occur at longer MRTs and lead to the disconnect between taxonomic and functional diversity profiles.
Identifiants
pubmed: 30787397
doi: 10.1038/s41396-019-0371-6
pii: 10.1038/s41396-019-0371-6
pmc: PMC6544533
mid: EMS81648
doi:
Substances chimiques
DNA, Bacterial
0
RNA, Ribosomal, 16S
0
Sewage
0
Waste Water
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1589-1601Subventions
Organisme : European Research Council
ID : 614768
Pays : International
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