Screening and identification of a non-peptide antagonist for the peptide hormone receptor in
Arabidopsis
/ drug effects
Arabidopsis Proteins
/ antagonists & inhibitors
Binding, Competitive
Gene Expression Regulation, Developmental
/ drug effects
Gene Expression Regulation, Plant
/ drug effects
High-Throughput Screening Assays
Intercellular Signaling Peptides and Proteins
/ genetics
Meristem
/ drug effects
Plant Growth Regulators
/ chemistry
Plant Shoots
/ drug effects
Protein Binding
/ drug effects
Protein Serine-Threonine Kinases
/ antagonists & inhibitors
Seedlings
/ drug effects
Signal Transduction
Small Molecule Libraries
/ chemistry
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
2019
2019
Historique:
received:
13
06
2018
accepted:
15
01
2019
entrez:
23
2
2019
pubmed:
23
2
2019
medline:
23
2
2019
Statut:
epublish
Résumé
Intercellular signaling mediated by peptide hormones and membrane-localized receptor kinases plays crucial roles in plant developmental processes. Because of their diverse functions, agonistic or antagonistic modulation of peptide signaling holds enormous promise for agricultural applications. Here we established a high-throughput screening system using a bead-immobilized receptor kinase and fluorescent-labeled peptide ligand to identify small molecules that bind peptide hormone receptors in competition with natural ligands. We used the
Identifiants
pubmed: 30793040
doi: 10.1038/s42003-019-0307-8
pii: 10.1038/s42003-019-0307-8
pmc: PMC6377654
doi:
Substances chimiques
AT1G26600 protein, Arabidopsis
0
AT2G27250 protein, Arabidopsis
0
Arabidopsis Proteins
0
Intercellular Signaling Peptides and Proteins
0
Plant Growth Regulators
0
Small Molecule Libraries
0
BAM1 protein, Arabidopsis
EC 2.7.11.1
CLV1 protein, Arabidopsis
EC 2.7.11.1
Protein Serine-Threonine Kinases
EC 2.7.11.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Pagination
61Déclaration de conflit d'intérêts
The authors declare no competing interests.
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