An RNA aptamer to HP1/Swi6 facilitates heterochromatin formation at an ectopic locus in S.pombe.
None
Apatmer
SELEX
Swi6
heterochromatin
Journal
RNA biology
ISSN: 1555-8584
Titre abrégé: RNA Biol
Pays: United States
ID NLM: 101235328
Informations de publication
Date de publication:
06 2019
06 2019
Historique:
pubmed:
23
2
2019
medline:
18
12
2019
entrez:
23
2
2019
Statut:
ppublish
Résumé
In the fission yeast Schizosaccharomyces pombe (S.pombe), heterochromatin domains are established and maintained by protein complexes that contain numerous RNA binding domains among their components. The fission yeast HP1 protein Swi6 is one such component and contains an unstructured RNA-binding hinge, which is important for the integrity and silencing of heterochromatin. In this study, we have used an RNA aptamer that likely binds to the Swi6 hinge with high affinity, as a tool to perturb the natural interactions mediated by this domain. When the hinge is blocked by the aptamer RNA, Swi6 appears to become less restricted to the pericentromeres and is enriched at specific euchromatic loci. This suggests a role for the Swi6 hinge, along with the chromoshadow domain (previously shown) in controlling the spread of heterochromatin in S.pombe. The study also highlights the potential of using a synthetic aptamer RNA as a tool to perturb nucleic acid - protein interaction in vivo with the objective of understanding the functional relevance of such an interaction.
Identifiants
pubmed: 30794054
doi: 10.1080/15476286.2019.1584026
pmc: PMC6546410
doi:
Substances chimiques
Aptamers, Nucleotide
0
Chromosomal Proteins, Non-Histone
0
Heterochromatin
0
Schizosaccharomyces pombe Proteins
0
Swi6 protein, S pombe
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
742-753Références
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