The Eno Gene of Burkholderia cenocepacia Strain 71-2 is Involved in Phosphate Solubilization.
Bacterial Proteins
/ genetics
Burkholderia cenocepacia
/ classification
DNA, Bacterial
/ genetics
Genetic Complementation Test
Mutagenesis
Mutation
Phosphates
/ metabolism
Phosphopyruvate Hydratase
/ genetics
Phylogeny
RNA, Ribosomal, 16S
/ genetics
Rec A Recombinases
/ genetics
Rhizosphere
Sequence Analysis, DNA
Soil Microbiology
Nicotiana
/ microbiology
Journal
Current microbiology
ISSN: 1432-0991
Titre abrégé: Curr Microbiol
Pays: United States
ID NLM: 7808448
Informations de publication
Date de publication:
Apr 2019
Apr 2019
Historique:
received:
14
10
2018
accepted:
29
01
2019
pubmed:
25
2
2019
medline:
13
7
2019
entrez:
25
2
2019
Statut:
ppublish
Résumé
Bacterial strain 71-2 with phosphate-solubilizing activity was isolated from tobacco rhizosphere and classified as Burkholderia cenocepacia based on sequence analyses of 16S rRNA and recA genes. To learn phosphate-solubilizing mechanisms of 71-2, mutants showing reduced solubilizing phosphate activity were obtained using the EZ-Tn5 transposon. Mutant 71-2-MT51 was reduced in the solubilizing phosphate content to 34.36% as compared with the wild-type strain 71-2. The disrupted gene in 71-2-MT51 was cloned and sequenced, and the putative protein from the gene shared 65.26% identity to protein sequences of enolase from Escherichia coli, which suggests the gene encodes an enzyme of enolase. Complementation analyzing showed that Eno was responsible for phosphate solubilizing for B. cenocepacia strain 71-2. To our knowledge, this is the first report of Eno involved in phosphate solubilizing in B. cenocepacia as well as in other bacteria.
Identifiants
pubmed: 30798378
doi: 10.1007/s00284-019-01642-7
pii: 10.1007/s00284-019-01642-7
doi:
Substances chimiques
Bacterial Proteins
0
DNA, Bacterial
0
Phosphates
0
RNA, Ribosomal, 16S
0
Rec A Recombinases
EC 2.7.7.-
Phosphopyruvate Hydratase
EC 4.2.1.11
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
495-502Subventions
Organisme : Agro-Industry Research and Development Special Fund of China
ID : 2017YFD0201705
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