Genotypic diversity among Shewanella spp. collected from freshwater fish.
16S rRNA
ERIC-PCR
PFGE
Shewanella spp.
fish
genotyping
Journal
Journal of fish diseases
ISSN: 1365-2761
Titre abrégé: J Fish Dis
Pays: England
ID NLM: 9881188
Informations de publication
Date de publication:
May 2019
May 2019
Historique:
received:
26
11
2018
revised:
09
01
2019
accepted:
10
01
2019
pubmed:
28
2
2019
medline:
11
5
2019
entrez:
28
2
2019
Statut:
ppublish
Résumé
Different Shewanella species are isolated both from healthy and from diseased fish. To date, contemporary methods do not provide sufficient insight to determine species and detail differentiation between tested strains. Bacteria isolated from cultured (n = 33), wild (n = 12) and ornamental (n = 6) fish, as well as several reference strains, were tested by 16S rRNA gene sequencing, ERIC-PCR and pulsed-field gel electrophoresis (PFGE) assays. Our study indicates that isolates collected from freshwater fish were genetically diverse. Based on 16S rRNA gene sequences, bacteria were clustered into groups S. putrefaciens, S. xiamenensis and S. oneidensis. Some isolates were classified only to genus Shewanella; thus, 16S rRNA gene analyses were not enough to determine the species. ERIC-PCR revealed 49 different genotype profiles indicating that the method might be useful for differentiation of Shewanella isolates irrespectively to species identification, contrary to PFGE which is not suitable for Shewanella typing.
Substances chimiques
RNA, Bacterial
0
RNA, Ribosomal, 16S
0
Banques de données
GENBANK
['KC607503', 'KC607522', 'KC607524', 'KC607531', 'KY630559', 'KY817247', 'KY817258', 'MF958276', 'MF958964', 'MF959701', 'MF962592', 'MF962594', 'MF962596', 'MF962598', 'MG930082', 'MH169228']
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
677-684Subventions
Organisme : National Science Centre, Poland
ID : 2015/19/N/NZ7/01687
Informations de copyright
© 2019 John Wiley & Sons Ltd.