A dynamically interacting flexible loop assists oligomerisation of the Caenorhabditis elegans centriolar protein SAS-6.
Amino Acid Sequence
Animals
Caenorhabditis elegans
/ metabolism
Caenorhabditis elegans Proteins
/ chemistry
Cell Cycle Proteins
/ chemistry
Centrioles
/ metabolism
Crystallography, X-Ray
Humans
Molecular Dynamics Simulation
Nuclear Magnetic Resonance, Biomolecular
Protein Domains
Protein Multimerization
Protein Structure, Tertiary
Recombinant Proteins
/ biosynthesis
Sequence Alignment
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
05 03 2019
05 03 2019
Historique:
received:
04
10
2018
accepted:
11
02
2019
entrez:
7
3
2019
pubmed:
7
3
2019
medline:
22
9
2020
Statut:
epublish
Résumé
Centrioles are conserved organelles fundamental for the organisation of microtubules in animal cells. Oligomerisation of the spindle assembly abnormal protein 6 (SAS-6) is an essential step in the centriole assembly process and may act as trigger for the formation of these organelles. SAS-6 oligomerisation is driven by two independent interfaces, comprising an extended coiled coil and a dimeric N-terminal globular domain. However, how SAS-6 oligomerisation is controlled remains unclear. Here, we show that in the Caenorhabditis elegans SAS-6, a segment of the N-terminal globular domain, unresolved in crystallographic structures, comprises a flexible loop that assists SAS-6 oligomerisation. Atomistic molecular dynamics simulations and nuclear magnetic resonance experiments suggest that transient interactions of this loop across the N-terminal dimerisation interface stabilise the SAS-6 oligomer. We discuss the possibilities presented by such flexible SAS-6 segments for the control of centriole formation.
Identifiants
pubmed: 30837637
doi: 10.1038/s41598-019-40294-2
pii: 10.1038/s41598-019-40294-2
pmc: PMC6401066
doi:
Substances chimiques
Caenorhabditis elegans Proteins
0
Cell Cycle Proteins
0
Recombinant Proteins
0
SAS-6 protein, C elegans
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3526Subventions
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/N009274/1
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 094872/Z/10/Z
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/J008265/1
Pays : United Kingdom
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