Tandem Fluorescent Protein Timers for Noninvasive Relative Protein Lifetime Measurement in Plants.
Journal
Plant physiology
ISSN: 1532-2548
Titre abrégé: Plant Physiol
Pays: United States
ID NLM: 0401224
Informations de publication
Date de publication:
06 2019
06 2019
Historique:
received:
15
01
2019
accepted:
08
03
2019
pubmed:
16
3
2019
medline:
25
8
2020
entrez:
16
3
2019
Statut:
ppublish
Résumé
Targeted protein degradation is an important and pervasive regulatory mechanism in plants, required for perception and response to the environment as well as developmental signaling. Despite the significance of this process, relatively few studies have assessed plant protein turnover in a quantitative fashion. Tandem fluorescent protein timers (tFTs) offer a powerful approach for the assessment of in vivo protein turnover in distinct subcellular compartments of single or multiple cells. A tFT is a fusion of two different fluorescent proteins with distinct fluorophore maturation kinetics, which enable protein age to be estimated from the ratio of fluorescence intensities of the two fluorescent proteins. Here, we used short-lived auxin signaling proteins and model N-end rule (N-recognin) pathway reporters to demonstrate the utility of tFTs for studying protein turnover in living plant cells of Arabidopsis (
Identifiants
pubmed: 30872425
pii: pp.19.00051
doi: 10.1104/pp.19.00051
pmc: PMC6548237
doi:
Substances chimiques
Indoleacetic Acids
0
Luminescent Proteins
0
Plant Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
718-731Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/J016276/1
Pays : United Kingdom
Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2019 American Society of Plant Biologists. All Rights Reserved.
Références
Nat Cell Biol. 2005 Nov;7(11):1057-65
pubmed: 16244669
Plant Cell. 2001 Apr;13(4):829-41
pubmed: 11283339
Cell. 2018 Mar 22;173(1):260-274.e25
pubmed: 29551266
Plant Cell. 2017 Feb;29(2):207-228
pubmed: 28138016
Proc Natl Acad Sci U S A. 2009 Aug 11;106(32):13618-23
pubmed: 19620738
New Phytol. 2016 Sep;211(4):1188-94
pubmed: 27439310
Plant Physiol. 2005 Jun;138(2):624-35
pubmed: 15955926
New Phytol. 2014 Jul;203(2):362-77
pubmed: 24863651
J Exp Bot. 2015 Feb;66(4):1133-44
pubmed: 25635110
Science. 1986 Oct 10;234(4773):179-86
pubmed: 3018930
Trends Cell Biol. 2014 Oct;24(10):603-11
pubmed: 24874449
Nat Commun. 2015 Jul 17;6:7640
pubmed: 26184543
Philos Trans A Math Phys Eng Sci. 2016 Oct 28;374(2079):
pubmed: 27644981
Physiol Plant. 2008 Jun;133(2):397-405
pubmed: 18298415
Plant J. 1998 Dec;16(6):735-43
pubmed: 10069079
Plant Cell. 2001 Mar;13(3):465-80
pubmed: 11251090
Nature. 2011 Oct 23;479(7373):419-22
pubmed: 22020282
Proc Natl Acad Sci U S A. 1998 Jul 7;95(14):7904-8
pubmed: 9653113
PLoS Biol. 2008 Dec 16;6(12):e307
pubmed: 19090618
Protein Sci. 2011 Aug;20(8):1298-345
pubmed: 21633985
Nat Commun. 2012;3:1204
pubmed: 23149748
J Proteome Res. 2013 Jul 5;12(7):3449-59
pubmed: 23750852
Plant Physiol. 2003 Nov;133(3):1360-6
pubmed: 14551326
Nat Biotechnol. 2012 Jun 24;30(7):708-14
pubmed: 22729030
New Phytol. 2018 Jan;217(2):613-624
pubmed: 28277608
Science. 1998 Feb 27;279(5355):1371-3
pubmed: 9478901
J Proteome Res. 2009 Jan;8(1):104-12
pubmed: 18954100
Plant Cell. 2001 Oct;13(10):2349-60
pubmed: 11595806
Mol Cell. 2018 Sep 20;71(6):1079-1091.e9
pubmed: 30146318
New Phytol. 2018 May;218(3):1106-1126
pubmed: 29168982
Nat Chem Biol. 2012 Apr 01;8(5):477-85
pubmed: 22466420
Plant J. 2000 Mar;21(6):553-62
pubmed: 10758506
Plant Physiol. 2015 Sep;169(1):803-13
pubmed: 26149575
Methods Enzymol. 2000;327:578-93
pubmed: 11045010
Mol Cell. 2018 May 3;70(3):488-501.e5
pubmed: 29727619
Plant Physiol. 2007 Dec;145(4):1183-91
pubmed: 17965171
J Exp Bot. 2015 Apr;66(8):2155-65
pubmed: 25547917
ACS Chem Biol. 2012 Aug 17;7(8):1444-53
pubmed: 22716912
Nature. 2001 Nov 15;414(6861):271-6
pubmed: 11713520
Trends Plant Sci. 2001 Sep;6(9):420-5
pubmed: 11544131
Proc Natl Acad Sci U S A. 2003 Mar 4;100(5):2987-91
pubmed: 12594336
Proc Natl Acad Sci U S A. 2006 Apr 11;103(15):6055-60
pubmed: 16581911
Curr Opin Plant Biol. 2018 Oct;45(Pt A):18-25
pubmed: 29775763
Nature. 2013 Nov 14;503(7475):285-9
pubmed: 24067609
Trends Plant Sci. 2005 Mar;10(3):103-5
pubmed: 15749466
Proc Natl Acad Sci U S A. 1994 Jan 4;91(1):326-30
pubmed: 8278386
Mol Syst Biol. 2018 Sep 4;14(9):e8355
pubmed: 30181144
Plant Cell. 2006 Mar;18(3):699-714
pubmed: 16489122
J Biol Chem. 2010 Oct 22;285(43):32810-7
pubmed: 20720017
Annu Rev Cell Dev Biol. 2014;30:207-33
pubmed: 25288113
Open Biol. 2014 Apr 16;4:140002
pubmed: 24740984
Annu Rev Biochem. 2012;81:379-405
pubmed: 22439968
Methods Mol Biol. 2014;1174:195-210
pubmed: 24947383
Nature. 2005 May 26;435(7041):446-51
pubmed: 15917798
Proc Natl Acad Sci U S A. 2000 Oct 24;97(22):11990-5
pubmed: 11050230
Methods Mol Biol. 2014;1056:11-7
pubmed: 24306858
Bioorg Med Chem. 2013 Jun 15;21(12):3400-10
pubmed: 23485445
Plant J. 1996 Sep;10(3):403-13
pubmed: 8811856
Trends Plant Sci. 2002 May;7(5):193-5
pubmed: 11992820
Dev Cell. 2005 Jul;9(1):109-19
pubmed: 15992545
Nat Plants. 2015 Mar 03;1:15017
pubmed: 27246884
Eur J Cell Biol. 2010 Feb-Mar;89(2-3):231-5
pubmed: 19944476
Mol Biol Cell. 2016 Jan 15;27(2):360-70
pubmed: 26609072
Nature. 2012 Jan 15;482(7383):103-6
pubmed: 22246322
Curr Biol. 2017 Oct 23;27(20):3183-3190.e4
pubmed: 29033328
Plant Cell. 2005 Aug;17(8):2369-83
pubmed: 15994909
Mol Cell Proteomics. 2016 Dec;15(12):3551-3563
pubmed: 27765818
Mol Cell Proteomics. 2012 Dec;11(12):1551-65
pubmed: 23125033
J Exp Bot. 2018 Aug 31;69(19):4555-4568
pubmed: 29945174
Development. 2016 Jan 1;143(1):174-9
pubmed: 26603383
Proteomics. 2014 Mar;14(4-5):579-92
pubmed: 24323582
Methods Mol Biol. 2014;1062:165-73
pubmed: 24057365
Nat Protoc. 2006;1(4):2019-25
pubmed: 17487191
Nat Rev Mol Cell Biol. 2009 Jun;10(6):385-97
pubmed: 19424292
Nature. 2005 May 26;435(7041):441-5
pubmed: 15917797
Nature. 2011 May 19;473(7347):337-42
pubmed: 21593866
Nature. 2011 Oct 23;479(7373):415-8
pubmed: 22020279
FEBS Lett. 2007 Jul 10;581(17):3189-96
pubmed: 17572409
J Cell Sci. 2018 Feb 7;131(3):
pubmed: 29361529
Curr Biol. 2010 Oct 12;20(19):1697-706
pubmed: 20888232
Mol Cell Proteomics. 2012 Mar;11(3):M111.011429
pubmed: 21937730
Science. 2010 Feb 19;327(5968):973-7
pubmed: 20110468
J Exp Bot. 2018 Aug 31;69(19):4511-4527
pubmed: 29726957
PLoS One. 2012;7(8):e43414
pubmed: 22912871
Development. 1999 Feb;126(4):711-21
pubmed: 9895319
Anal Biochem. 2007 Sep 1;368(1):95-9
pubmed: 17586454
Science. 2011 Feb 11;331(6018):764-8
pubmed: 21233346
Annu Rev Plant Biol. 2011;62:299-334
pubmed: 21370976
Plant Cell. 2015 May;27(5):1547-62
pubmed: 25966763