Sulfide (Na₂S) and Polysulfide (Na₂S₂) Interacting with Doxycycline Produce/Scavenge Superoxide and Hydroxyl Radicals and Induce/Inhibit DNA Cleavage.
DNA cleavage
EPR spectroscopy
doxycycline
hydrogen sulfide
hydroxyl radical
oxytetracycline
polysulfides
superoxide
tetracycline
•cPTIO radical
Journal
Molecules (Basel, Switzerland)
ISSN: 1420-3049
Titre abrégé: Molecules
Pays: Switzerland
ID NLM: 100964009
Informations de publication
Date de publication:
22 Mar 2019
22 Mar 2019
Historique:
received:
22
02
2019
revised:
15
03
2019
accepted:
19
03
2019
entrez:
27
3
2019
pubmed:
27
3
2019
medline:
16
7
2019
Statut:
epublish
Résumé
Doxycycline (DOXY) is an antibiotic routinely prescribed in human and veterinary medicine for antibacterial treatment, but it has also numerous side effects that include oxidative stress, inflammation, cancer or hypoxia-induced injury. Endogenously produced hydrogen sulfide (H₂S) and polysulfides affect similar biological processes, in which reactive oxygen species (ROS) play a role. Herein, we have studied the interaction of DOXY with H₂S (Na₂S) or polysulfides (Na₂S₂, Na₂S₃ and Na₂S₄) to gain insights into the biological effects of intermediates/products that they generate. To achieve this, UV-VIS, EPR spectroscopy and plasmid DNA (pDNA) cleavage assay were employed. Na₂S or Na₂S₂ in a mixture with DOXY, depending on ratio, concentration and time, displayed bell-shape kinetics in terms of producing/scavenging superoxide and hydroxyl radicals and decomposing hydrogen peroxide. In contrast, the effects of individual compounds (except for Na₂S₂) were hardly observable. In addition, DOXY, as well as oxytetracycline and tetracycline, interacting with Na₂S or other studied polysulfides reduced the
Identifiants
pubmed: 30909480
pii: molecules24061148
doi: 10.3390/molecules24061148
pmc: PMC6470963
pii:
doi:
Substances chimiques
Sulfides
0
Superoxides
11062-77-4
Hydroxyl Radical
3352-57-6
polysulfide
9080-49-3
Doxycycline
N12000U13O
sodium sulfide
YGR27ZW0Y7
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Agentúra na Podporu Výskumu a Vývoja
ID : 15-0371; 17-0384; 15-0565
Organisme : Vedecká Grantová Agentúra MŠVVaŠ SR a SAV
ID : 2/0079/19; 1/0041/15; 2/0053/19; 2/0014/17
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