Single-cell chromatin immunocleavage sequencing (scChIC-seq) to profile histone modification.
Animals
Antibodies
/ chemistry
Chromatin
/ chemistry
Chromatin Immunoprecipitation
Computational Biology
DNA
/ chemistry
Epigenomics
Genome
High-Throughput Nucleotide Sequencing
Histone Code
Histones
/ chemistry
Humans
Leukocytes
/ cytology
Male
Mice
Micrococcal Nuclease
/ chemistry
NIH 3T3 Cells
Nucleosomes
Polymerase Chain Reaction
Promoter Regions, Genetic
Protein Processing, Post-Translational
Reproducibility of Results
Sequence Analysis, DNA
Software
Journal
Nature methods
ISSN: 1548-7105
Titre abrégé: Nat Methods
Pays: United States
ID NLM: 101215604
Informations de publication
Date de publication:
04 2019
04 2019
Historique:
received:
25
10
2017
accepted:
20
02
2019
entrez:
30
3
2019
pubmed:
30
3
2019
medline:
30
5
2019
Statut:
ppublish
Résumé
Our method for analyzing histone modifications, scChIC-seq (single-cell chromatin immunocleavage sequencing), involves targeting of the micrococcal nuclease (MNase) to a histone mark of choice by tethering to a specific antibody. Cleaved target sites are then selectively PCR amplified. We show that scChIC-seq reliably detects H3K4me3 and H3K27me3 target sites in single human white blood cells. The resulting data are used for clustering of blood cell types.
Identifiants
pubmed: 30923384
doi: 10.1038/s41592-019-0361-7
pii: 10.1038/s41592-019-0361-7
pmc: PMC7187538
mid: NIHMS1568006
doi:
Substances chimiques
Antibodies
0
Chromatin
0
Histones
0
Nucleosomes
0
histone H3 trimethyl Lys4
0
DNA
9007-49-2
Micrococcal Nuclease
EC 3.1.31.1
Types de publication
Journal Article
Research Support, N.I.H., Intramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
323-325Subventions
Organisme : Intramural NIH HHS
ID : ZIA HL005801
Pays : United States
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