Clonal expansion of community-associated meticillin-resistant Staphylococcus aureus (MRSA) in people who inject drugs (PWID): prevalence, risk factors and molecular epidemiology, Bristol, United Kingdom, 2012 to 2017.

Community acquired infections Community acquired infections epidemiology Community acquired infections microbiology Drug users Injecting drug use Intravenous Intravenous: complications Intravenous: microbiology MRSA Meticillin-Resistant Staphylococcus aureus Sepsis Sequence Analysis, DNA Staphylococcal Infections/transmission Staphylococcal epidemiology Substance Abuse Substance-Related Disorders/complications Substance-Related Disorders/microbiology United Kingdom Whole-genome sequencing

Journal

Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin
ISSN: 1560-7917
Titre abrégé: Euro Surveill
Pays: Sweden
ID NLM: 100887452

Informations de publication

Date de publication:
Mar 2019
Historique:
entrez: 4 4 2019
pubmed: 4 4 2019
medline: 16 7 2020
Statut: ppublish

Résumé

In 2015, Bristol (South West England) experienced a large increase in cases of meticillin-resistant We aimed to characterise and estimate the prevalence of MRSA colonisation among PWID in Bristol and test evidence of a clonal outbreak. PWID recruited through an unlinked-anonymous community survey during 2016 completed behavioural questionnaires and were screened for MRSA. Univariable logistic regression examined associations with MRSA colonisation. Whole-genome sequencing used lineage-matched MRSA isolates, comparing PWID (screening and retrospective bacteraemia samples from 2012-2017) with non-PWID (Bristol screening) in Bristol and national reference laboratory database samples. The MRSA colonisation prevalence was 8.7% (13/149) and was associated with frequently injecting in public places (odds ratio (OR): 5.5; 95% confidence interval (CI):1.34-22.70), recent healthcare contact (OR: 4.3; 95% CI: 1.34-13.80) and injecting in groups of three or more (OR: 15.8; 95% CI: 2.51-99.28). People reporting any one of: injecting in public places, injection site skin and soft tissue infection or hospital contact accounted for 12/13 MRSA positive cases (sensitivity 92.3%; specificity 51.5%). Phylogenetic analysis identified a dominant clade associated with infection and colonisation among PWID in Bristol belonging to ST5-SCCmecIVg. MRSA colonisation in Bristol PWID is substantially elevated compared with general population estimates and there is evidence of clonal expansion, community-based transmission and increased infection risk related to the colonising strain. Targeted interventions, including community screening and suppression therapy, education and basic infection control are needed to reduce MRSA infections in PWID.

Sections du résumé

BACKGROUND BACKGROUND
In 2015, Bristol (South West England) experienced a large increase in cases of meticillin-resistant
AIM OBJECTIVE
We aimed to characterise and estimate the prevalence of MRSA colonisation among PWID in Bristol and test evidence of a clonal outbreak.
METHODS METHODS
PWID recruited through an unlinked-anonymous community survey during 2016 completed behavioural questionnaires and were screened for MRSA. Univariable logistic regression examined associations with MRSA colonisation. Whole-genome sequencing used lineage-matched MRSA isolates, comparing PWID (screening and retrospective bacteraemia samples from 2012-2017) with non-PWID (Bristol screening) in Bristol and national reference laboratory database samples.
RESULTS RESULTS
The MRSA colonisation prevalence was 8.7% (13/149) and was associated with frequently injecting in public places (odds ratio (OR): 5.5; 95% confidence interval (CI):1.34-22.70), recent healthcare contact (OR: 4.3; 95% CI: 1.34-13.80) and injecting in groups of three or more (OR: 15.8; 95% CI: 2.51-99.28). People reporting any one of: injecting in public places, injection site skin and soft tissue infection or hospital contact accounted for 12/13 MRSA positive cases (sensitivity 92.3%; specificity 51.5%). Phylogenetic analysis identified a dominant clade associated with infection and colonisation among PWID in Bristol belonging to ST5-SCCmecIVg.
CONCLUSIONS CONCLUSIONS
MRSA colonisation in Bristol PWID is substantially elevated compared with general population estimates and there is evidence of clonal expansion, community-based transmission and increased infection risk related to the colonising strain. Targeted interventions, including community screening and suppression therapy, education and basic infection control are needed to reduce MRSA infections in PWID.

Identifiants

pubmed: 30940316
doi: 10.2807/1560-7917.ES.2019.24.13.1800124
pmc: PMC6446509
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/K023233/1
Pays : United Kingdom

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Auteurs

Simon Packer (S)

Field Epidemiology Service, Public Health England, Bristol, United Kingdom.

Bruno Pichon (B)

Antimicrobial Resistance and Healthcare Associated Infections Reference Unit, National Infection Service, Public Health England, London, United Kingdom.

Stephen Thompson (S)

Public health laboratory Bristol, Public Health England, Bristol, United Kingdom.

Jane Neale (J)

Bristol Drugs Project, Bristol, United Kingdom.

Jacquelyn Njoroge (J)

Blood Borne Virus Section, HIV & STI Department, National Infection Service, Public Health England, London, United Kingdom.

Rachel M Kwiatkowska (RM)

Field Epidemiology Service, Public Health England, Bristol, United Kingdom.
NIHR Health Protection Research Unit in Evaluation of Interventions at University of Bristol, Bristol, United Kingdom.

Isabel Oliver (I)

Field Epidemiology Service, Public Health England, Bristol, United Kingdom.
NIHR Health Protection Research Unit in Evaluation of Interventions at University of Bristol, Bristol, United Kingdom.

Maggie Telfer (M)

Bristol Drugs Project, Bristol, United Kingdom.

Michel Doumith (M)

Antimicrobial Resistance and Healthcare Associated Infections Reference Unit, National Infection Service, Public Health England, London, United Kingdom.
Infectious Diseases Research Department, King Abdullah International Medical Research Center, Riyadh, Saudi Arabia.
King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia.

Camillus Buunaaisie (C)

University of West England, Bristol, United Kingdom.

Ellen Heinsbroek (E)

Blood Borne Virus Section, HIV & STI Department, National Infection Service, Public Health England, London, United Kingdom.

Noreen Hopewell-Kelly (N)

University of West England, Bristol, United Kingdom.

Monica Desai (M)

Blood Borne Virus Section, HIV & STI Department, National Infection Service, Public Health England, London, United Kingdom.

Vivian Hope (V)

Public Health Institute, Liverpool John Moores University, Liverpool, United Kingdom.
Blood Borne Virus Section, HIV & STI Department, National Infection Service, Public Health England, London, United Kingdom.

Owen Martin Williams (OM)

Public health laboratory Bristol, Public Health England, Bristol, United Kingdom.

Angela Kearns (A)

Authors contributed equally to the work and share last authorship.
Antimicrobial Resistance and Healthcare Associated Infections Reference Unit, National Infection Service, Public Health England, London, United Kingdom.

Mathew Hickman (M)

School of Social and Community Medicine, University of Bristol, Bristol, United Kingdom.
Authors contributed equally to the work and share last authorship.
NIHR Health Protection Research Unit in Evaluation of Interventions at University of Bristol, Bristol, United Kingdom.

Maya Gobin (M)

Field Epidemiology Service, Public Health England, Bristol, United Kingdom.
Authors contributed equally to the work and share last authorship.

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