Beneficial Effects of the Angiotensin-Converting Enzyme 2 Activator Dize in Renovascular Hypertension.
Acetylglucosaminidase
/ metabolism
Angiotensin I
/ metabolism
Angiotensin-Converting Enzyme 2
Animals
Cytokines
/ metabolism
Diminazene
/ analogs & derivatives
Enzyme Activators
/ pharmacology
Hypertension, Renovascular
/ drug therapy
Inflammation
/ drug therapy
Kidney
/ drug effects
Male
Nitric Oxide
/ metabolism
Peptide Fragments
/ metabolism
Peptidyl-Dipeptidase A
/ metabolism
Peroxidase
/ metabolism
Rats, Wistar
Renin-Angiotensin System
/ drug effects
ACE2 activation
angiotensin-(1-7)
diminazene aceturate
renin
angiotensin system
renoprotection
renovascular hypertension.
Journal
Protein and peptide letters
ISSN: 1875-5305
Titre abrégé: Protein Pept Lett
Pays: Netherlands
ID NLM: 9441434
Informations de publication
Date de publication:
2019
2019
Historique:
received:
17
01
2019
revised:
22
03
2019
accepted:
23
03
2019
pubmed:
6
4
2019
medline:
8
10
2019
entrez:
6
4
2019
Statut:
ppublish
Résumé
Angiotensin Converting Enzyme (ACE) 2 is an important modulator of the Renin Angiotensin System (RAS) and the RAS plays a central role in renovascular hypertension. Very few studies investigated the role of components of the counterregulatory RAS axis (ACE2, Ang-(1-7) and Mas receptor) in renovascular hypertension and the results are controversial. The aim of this study was to investigate the effects of Diminazene Aceturate (DIZE) administration on renal function and renal inflammation parameters in 2K1C hypertensive rats. Male Wistar rats were divided into three experimental groups: sham-operated animals, 2K1C+saline and 2K1C+DIZE orally (1 mg/kg/day). At the end of the 30 days of treatment, renal function was analyzed and kidneys from all the groups were collected and processed separately for measurement of N-acetyl-beta-D-glucosaminidase (NAG) and Myeloperoxidase (MPO) activities, cytokines, chemokines and nitric oxide levels. Oral DIZE administration for 4 weeks in hypertensive rats attenuated renal dysfunction and reduced the levels of MPO and NAG, cytokines and chemokines (IL1β, IL-6, TNF-α and MCP-1) and increased urinary nitrate/nitrite levels in 2K1C hypertensive rats. Our findings showed that ACE2 activation may effectively improve renal alterations and inflammation induced by renovascular hypertension.
Sections du résumé
BACKGROUND
BACKGROUND
Angiotensin Converting Enzyme (ACE) 2 is an important modulator of the Renin Angiotensin System (RAS) and the RAS plays a central role in renovascular hypertension. Very few studies investigated the role of components of the counterregulatory RAS axis (ACE2, Ang-(1-7) and Mas receptor) in renovascular hypertension and the results are controversial.
OBJECTIVE
OBJECTIVE
The aim of this study was to investigate the effects of Diminazene Aceturate (DIZE) administration on renal function and renal inflammation parameters in 2K1C hypertensive rats.
METHODS
METHODS
Male Wistar rats were divided into three experimental groups: sham-operated animals, 2K1C+saline and 2K1C+DIZE orally (1 mg/kg/day). At the end of the 30 days of treatment, renal function was analyzed and kidneys from all the groups were collected and processed separately for measurement of N-acetyl-beta-D-glucosaminidase (NAG) and Myeloperoxidase (MPO) activities, cytokines, chemokines and nitric oxide levels.
RESULTS
RESULTS
Oral DIZE administration for 4 weeks in hypertensive rats attenuated renal dysfunction and reduced the levels of MPO and NAG, cytokines and chemokines (IL1β, IL-6, TNF-α and MCP-1) and increased urinary nitrate/nitrite levels in 2K1C hypertensive rats.
CONCLUSION
CONCLUSIONS
Our findings showed that ACE2 activation may effectively improve renal alterations and inflammation induced by renovascular hypertension.
Identifiants
pubmed: 30950337
pii: PPL-EPUB-97826
doi: 10.2174/0929866526666190405123422
doi:
Substances chimiques
Cytokines
0
Enzyme Activators
0
Peptide Fragments
0
Nitric Oxide
31C4KY9ESH
Angiotensin I
9041-90-1
Peroxidase
EC 1.11.1.7
Acetylglucosaminidase
EC 3.2.1.52
Peptidyl-Dipeptidase A
EC 3.4.15.1
Ace2 protein, rat
EC 3.4.17.23
Angiotensin-Converting Enzyme 2
EC 3.4.17.23
angiotensin I (1-7)
IJ3FUK8MOF
diminazene aceturate
JI8SAD85NO
Diminazene
Y5G36EEA5Z
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
523-531Informations de copyright
Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.