Ionic strength for tailoring the synthesis of monomodal stealth cationic liposomes in microfluidic devices.
Diffusion-based mass transfer
Hydrodynamic flow-focusing
Ionic strength
Lipid self-assembly
Micelles
Microfluidics
PEG
Stealth liposomes
Journal
Colloids and surfaces. B, Biointerfaces
ISSN: 1873-4367
Titre abrégé: Colloids Surf B Biointerfaces
Pays: Netherlands
ID NLM: 9315133
Informations de publication
Date de publication:
01 Jul 2019
01 Jul 2019
Historique:
received:
12
11
2018
revised:
23
03
2019
accepted:
25
03
2019
pubmed:
12
4
2019
medline:
21
11
2019
entrez:
12
4
2019
Statut:
ppublish
Résumé
In this work, we describe a hydrodynamic flow-focusing microfluidic process to produce stealth cationic liposomes (SCL), stabilized with poly(ethylene glycol) (PEG), with uniform and reproducible features. Through cryogenic transmission electron microscopy (cryo-TEM) characterization and real-time monitoring, we verified the formation of multi-sized lipid self-aggregates, which can be attributed to micelles formation. These structures tend to undergo deposition within the PDMS/glass microchannels through intermolecular interactions with the glass walls, hindering not only the process reproducibility but also the final biological application of the SCL products. In view of this, we propose the modulation of ionic strength of the side streams aiming to ionically shield the glass surface, decrease the intermolecular interactions of the lipid polar heads, and, essentially, to promote the bilayer-driven self-assembly of SCL with 1% of DSPE-PEG
Identifiants
pubmed: 30974261
pii: S0927-7765(19)30205-X
doi: 10.1016/j.colsurfb.2019.03.056
pii:
doi:
Substances chimiques
1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-methoxy-poly(ethylene glycol 2000)
0
Cations
0
Liposomes
0
Phosphatidylethanolamines
0
Polyethylene Glycols
3WJQ0SDW1A
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
233-241Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.