Tailoring the CRISPR system to transactivate coagulation gene promoters in normal and mutated contexts.
Binding Sites
Biomarkers, Tumor
/ genetics
CRISPR-Cas Systems
Carcinoma, Hepatocellular
Endothelial Cells
Factor VII
Factor VIII
/ genetics
Gene Expression
Genes, Reporter
Hep G2 Cells
Hepatocyte Nuclear Factor 4
/ genetics
Humans
Mutation
Promoter Regions, Genetic
/ genetics
RNA, Guide, Kinetoplastida
Receptors, Immunologic
/ genetics
Transcription Factors
/ genetics
Transcriptional Activation
/ genetics
CRISPR activation
Engineered transcription factors
Promoter mutations
TALE-TF
Journal
Biochimica et biophysica acta. Gene regulatory mechanisms
ISSN: 1876-4320
Titre abrégé: Biochim Biophys Acta Gene Regul Mech
Pays: Netherlands
ID NLM: 101731723
Informations de publication
Date de publication:
06 2019
06 2019
Historique:
received:
03
01
2019
revised:
26
03
2019
accepted:
11
04
2019
pubmed:
22
4
2019
medline:
16
10
2019
entrez:
22
4
2019
Statut:
ppublish
Résumé
Engineered transcription factors (TF) have expanded our ability to modulate gene expression and hold great promise as bio-therapeutics. The first-generation TF, based on Zinc Fingers or Transcription-Activator-like Effectors (TALE), required complex and time-consuming assembly protocols, and were indeed replaced in recent years by the CRISPR activation (CRISPRa) technology. Here, with coagulation F7/F8 gene promoters as models, we exploited a CRISPRa system based on deactivated (d)Cas9, fused with a transcriptional activator (VPR), which is driven to its target by a single guide (sg)RNA. Reporter gene assays in hepatoma cells identified a sgRNA (sgRNA
Identifiants
pubmed: 31005673
pii: S1874-9399(18)30536-4
doi: 10.1016/j.bbagrm.2019.04.002
pii:
doi:
Substances chimiques
Biomarkers, Tumor
0
Hepatocyte Nuclear Factor 4
0
RNA, Guide
0
Receptors, Immunologic
0
Transcription Factors
0
trophoblastic beta 1-glycoprotein receptor, human
0
F8 protein, human
839MOZ74GK
Factor VII
9001-25-6
Factor VIII
9001-27-8
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
619-624Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.