Characterization of a potent dominant negative mutant variant of Rad51 in Ustilago maydis.
BRCA2
DNA repair
Dominant negative
Homologous recombination
Rad51
Journal
DNA repair
ISSN: 1568-7856
Titre abrégé: DNA Repair (Amst)
Pays: Netherlands
ID NLM: 101139138
Informations de publication
Date de publication:
06 2019
06 2019
Historique:
received:
27
12
2018
revised:
08
03
2019
accepted:
09
04
2019
pubmed:
22
4
2019
medline:
9
1
2020
entrez:
22
4
2019
Statut:
ppublish
Résumé
Rad51 serves to maintain and protect integrity of the genome through its actions in DNA repair and replication fork protection. The active form of Rad51 is a nucleoprotein filament consisting of chains of protomer units arranged linearly along single-stranded DNA. In a mutant screen using Ustilago maydis as an experimental system we identified a novel variant of Rad51, in which an amino acid change near the protomer-protomer interaction interface confers a strong trans dominant inhibitory effect on resistance to DNA damaging agents and proficiency in homologous recombination. Modeling studies of the mutated residue D161Y suggested that steric interference with surrounding residues was the likely cause of the inhibitory effect. Changes of two nearby residues, predicted from the modeling to minimize steric clashes, mitigated the inhibition of DNA repair. Direct testing of purified Rad51
Identifiants
pubmed: 31005682
pii: S1568-7864(18)30333-1
doi: 10.1016/j.dnarep.2019.04.003
pii:
doi:
Substances chimiques
Mutant Proteins
0
Rad51 Recombinase
EC 2.7.7.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
91-101Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.