Synthesis of a Fluorescent Cytidine TNA Triphosphate Analogue.

DNA / chemistry DNA-Directed DNA Polymerase / metabolism Fluorescent Dyes / chemistry Nucleic Acids / chemistry Tetroses / chemical synthesis
1,3-diaza-2-oxo-phenothiazine Fluorescence Nucleoside triphosphate Threose nucleic acid (TNA) Xeno-nucleic acids (XNA)

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2019
Historique:
entrez: 25 4 2019
pubmed: 25 4 2019
medline: 7 9 2019
Statut: ppublish

Résumé

Threose nucleic acid (TNA) is refractory to nuclease digestion and capable of undergoing Darwinian evolution, which together make it a promising system for diagnostic and therapeutic applications that require high biological stability. Expanding the sequence/chemical diversity of TNA would enable the development of functional TNA molecules with enhanced physicochemical properties. Recently, we have reported the synthesis and polymerase activity of a fluorescent cytidine TNA triphosphate analogue (1,3-diaza-2-oxo-phenothiazine, tC

Identifiants

DOI: 10.1007/978-1-4939-9216-4_3 PMID: 31016694
pubmed: 31016694
doi: 10.1007/978-1-4939-9216-4_3
doi:

Substances chimiques

Fluorescent Dyes 0
Nucleic Acids 0
Tetroses 0
DNA 9007-49-2
DNA-Directed DNA Polymerase EC 2.7.7.7

Types de publication

Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

27-37

Auteurs

Hui Mei (H)

Department of Chemistry, Stanford University, Stanford, CA, USA. huimei@stanford.edu.

John Chaput (J)

Department of Pharmaceutical Sciences, University of California, Irvine, Irvine, CA, USA. jchaput@uci.edu.
Department of Chemistry, University of California, Irvine, Irvine, CA, USA. jchaput@uci.edu.
Department of Molecular Biology and Biochemistry, University of California, Irvine, Irvine, CA, USA. jchaput@uci.edu.

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Classifications MeSH

questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › Synthesis of a Fluorescent Cytidine TNA...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Transferases › Phosphotransferases › Nucleotidyltransferases › DNA nucleotidyltransferases › DNA-directed DNA polymerase › Synthesis of a Fluorescent Cytidine TNA...
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Produits chimiques de laboratoire › Indicateurs et réactifs › Luminescents › Colorants fluorescents › Synthesis of a Fluorescent Cytidine TNA...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › Synthesis of a Fluorescent Cytidine TNA...
questionsmedicales.fr › Glucides › Sucres › Oses › Tétroses › Synthesis of a Fluorescent Cytidine TNA...