NMR structure of a full-length single-pass membrane protein NRADD.

NRADD bicelles p75 neurotrophin receptor solution NMR structure

Journal

Proteins
ISSN: 1097-0134
Titre abrégé: Proteins
Pays: United States
ID NLM: 8700181

Informations de publication

Date de publication:
09 2019
Historique:
received: 17 09 2018
revised: 05 04 2019
accepted: 23 04 2019
pubmed: 30 4 2019
medline: 19 5 2020
entrez: 30 4 2019
Statut: ppublish

Résumé

Structural study of any single-pass membrane protein is both an important and challenging task. In this report, we present the structure of a neurotrophin receptor-alike death-domain protein. The structure and dynamics of the protein was investigated by conventional nuclear magnetic resonance techniques in the solution of phospholipid bicelles. The receptor contains two folded regions-α-helical transmembrane domain and globular C-terminal death domain with more than 50% of the rest of backbone being disordered. This is the first structure of a full-length single-pass membrane receptor-alike protein solved by the single method.

Identifiants

pubmed: 31033000
doi: 10.1002/prot.25703
doi:

Substances chimiques

Membrane Proteins 0
Phospholipids 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

786-790

Informations de copyright

© 2019 Wiley Periodicals, Inc.

Auteurs

Kirill D Nadezhdin (KD)

Laboratory of biomolecular NMR spectroscopy, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russia.
Department of biological and medical physics, Moscow Institute of Physics and Technology, Dolgoprudny, Russia.

Sergey A Goncharuk (SA)

Laboratory of biomolecular NMR spectroscopy, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russia.

Aleksander S Arseniev (AS)

Laboratory of biomolecular NMR spectroscopy, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russia.
Department of biological and medical physics, Moscow Institute of Physics and Technology, Dolgoprudny, Russia.

Konstantin S Mineev (KS)

Laboratory of biomolecular NMR spectroscopy, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russia.
Department of biological and medical physics, Moscow Institute of Physics and Technology, Dolgoprudny, Russia.

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Classifications MeSH