Effect of D-cysteine on dual-species biofilms of Streptococcus mutans and Streptococcus sanguinis.

Biofilms / drug effects Cysteine / metabolism Lactic Acid / metabolism Polysaccharides / metabolism Streptococcus mutans / drug effects Streptococcus sanguis / drug effects Virulence

Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
30 04 2019
Historique:
received: 12 10 2018
accepted: 12 04 2019
entrez: 2 5 2019
pubmed: 2 5 2019
medline: 21 10 2020
Statut: epublish

Résumé

Dental caries is a highly prevalent disease worldwide. It is caused by the cariogenic biofilms composed of multiple dynamic bacteria on dental surface. Streptococcus mutans and Streptococcus sanguinis are resident members within the biofilms and an antagonistic relationship has been shown between these two species. S. mutans, as the major causative microorganism of dental caries, has been reported to be inhibited by free D-cysteine (D-Cys). However, whether D-Cys could affect S. sanguinis and the interspecies relationship between S. mutans and S. sanguinis remains unknown. The aim of the current study was to investigate the effect of D-Cys on the growth and cariogenicity of dual-species biofilms formed by S. mutans and S. sanguinis. We measured dual-species biofilms biomass, metabolic activity, lactate production. We also detected the biofilms structure, the ratio of live/dead bacteria, extracellular polysaccharide (EPS) synthesis and bacterial composition in the dual-species biofilms. We found that D-Cys could reduce the metabolic activity and lactic acid production of dual-species biofilms (p < 0.05). In addition, biofilms formation, the proportion of S. mutans in dual-species biofilms, and EPS synthesis were decreased with D-Cys treatment. The results suggested that D-Cys could inhibit the growth and cariogenic virulence of dual-species biofilms formed by S. mutans and S. sanguinis, indicating the potential of D-Cys in clinical application for caries prevention and treatment.

Identifiants

DOI: 10.1038/s41598-019-43081-1 PMID: 31040318 PMC: PMC6491432
pubmed: 31040318
doi: 10.1038/s41598-019-43081-1
pii: 10.1038/s41598-019-43081-1
pmc: PMC6491432
doi:

Substances chimiques

Polysaccharides 0
Lactic Acid 33X04XA5AT
Cysteine K848JZ4886

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

6689

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Auteurs

Xiao Guo (X)

State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China.
Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China.

Shiyu Liu (S)

State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China.
Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China.

Xuedong Zhou (X)

State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China.
Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China.

Hongying Hu (H)

State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China.
Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China.

Keke Zhang (K)

Institute of Stem Cell and Tissue Engineering, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, 325035, China.

Xinmei Du (X)

State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China.

Xian Peng (X)

State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China.

Biao Ren (B)

State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China.
ORCID: 0000-0003-4215-2873

Lei Cheng (L)

State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China. chenglei@scu.edu.cn.
Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China. chenglei@scu.edu.cn.

Mingyun Li (M)

State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China. limingyun@scu.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Phénomènes microbiologiques › Biofilms › Effect of D-cysteine on dual-species biofilms...
questionsmedicales.fr › Acides aminés, peptides et protéines › Acides aminés › Acides aminés soufrés › Cystéine › Effect of D-cysteine on dual-species biofilms...
questionsmedicales.fr › Composés chimiques organiques › Acides carboxyliques › Hydroxyacides › Lactates › Acide lactique › Effect of D-cysteine on dual-species biofilms...
questionsmedicales.fr › Glucides › Polyosides › Effect of D-cysteine on dual-species biofilms...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Lactobacillales › Streptococcaceae › Streptococcus › Streptocoques viridans › Streptococcus mutans › Effect of D-cysteine on dual-species biofilms...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Lactobacillales › Streptococcaceae › Streptococcus › Streptocoques viridans › Streptococcus sanguis › Effect of D-cysteine on dual-species biofilms...
questionsmedicales.fr › Phénomènes microbiologiques › Virulence › Effect of D-cysteine on dual-species biofilms...