Myositis in Lewis rats induced by the superantigen Staphylococcal enterotoxin A.
Animal model
CD8+ T-lymphocytes
Microarray analysis
Myositis
Staphylococcal enterotoxin A (SEA)
Superantigen
Journal
Molecular biology reports
ISSN: 1573-4978
Titre abrégé: Mol Biol Rep
Pays: Netherlands
ID NLM: 0403234
Informations de publication
Date de publication:
Aug 2019
Aug 2019
Historique:
received:
19
03
2019
accepted:
03
05
2019
pubmed:
16
5
2019
medline:
15
2
2020
entrez:
16
5
2019
Statut:
ppublish
Résumé
The aetiology of inflammatory myopathies is not clearly known. A predominance of activated Cd8+ T lymphocytes in inflammatory infiltrates has already been detected. Superantigens activate lymphocytes in an oligoclonal manner. In the present investigation, we investigated local effects after injection of the superantigen (Sag) Staphylococcus enterotoxin A (SEA) in the quadriceps femoris muscle of Lewis rats. Histopathology and gene expression profiling was performed after injection of SEA or saline (control group) after one, three and 10 days. Histology revealed focal myositis predominated by Cd8+ T lymphocytes with a perimysial, endomysial and perivascular distribution, peaking 3 days after SEA injection. Using DNA microarray analysis (Affymetrix Rat Genome 230 2.0) genes that were differentially over-expressed at least 15 times at days one, three or ten after SEA injection were further analysed. One day after SEA injection over-expressed genes were related to the immune response (e.g. Fcnb, CD8a) but also to cell proliferation, differentiation and migration (e.g. Mpp2). Three days after SEA injection, differentially overexpressed genes were mainly related to the immune reaction with a clear signature for a Cd8+ T lymphocyte response (e.g. Cd3d, Cd8, Prf1, Gzmb). Ten days after SEA injection, the differentially overexpressed genes were again associated with the immune reaction (e.g. Cd3d, Il2) but also with regenerative processes and wound healing (e.g. Tgfa, Tpm1, Ripply1). The inflammatory response induced by SEA in Lewis rats shares histological and molecular similarities to polymyositis in humans. Therefore, SEA induced myositis can be taken as a new and apt model for polymyositis.
Identifiants
pubmed: 31087247
doi: 10.1007/s11033-019-04858-9
pii: 10.1007/s11033-019-04858-9
doi:
Substances chimiques
Enterotoxins
0
Histocompatibility Antigens Class II
0
Intracellular Signaling Peptides and Proteins
0
Membrane Proteins
0
Superantigens
0
enterotoxin A, Staphylococcal
37337-57-8
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
4085-4094Subventions
Organisme : Martin Luther University Halle-Wittenberg
ID : FKZ 28/45 Wilhelm-Roux program
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