Prokaryotic and viral community of the sulfate-rich crust from Peñahueca ephemeral lake, an astrobiology analogue.
Journal
Environmental microbiology
ISSN: 1462-2920
Titre abrégé: Environ Microbiol
Pays: England
ID NLM: 100883692
Informations de publication
Date de publication:
10 2019
10 2019
Historique:
received:
29
01
2019
revised:
09
05
2019
accepted:
11
05
2019
pubmed:
16
5
2019
medline:
13
5
2020
entrez:
16
5
2019
Statut:
ppublish
Résumé
Peñahueca is an athalassohaline hypersaline inland ephemeral lake originated under semiarid conditions in the central Iberian Peninsula (Spain). Its chemical composition makes it extreme for microbial life as well as a terrestrial analogue of other planetary environments. To investigate the persistence of microbial life associated with sulfate-rich crusts, we applied cultivation-independent methods (optical and electron microscopy, 16S rRNA gene profiling and metagenomics) to describe the prokaryotic community and its associated viruses. The diversity for Bacteria was very low and was vastly dominated by endospore formers related to Pontibacillus marinus of the Firmicutes phylum. The archaeal assemblage was more diverse and included taxa related to those normally found in hypersaline environments. Several 'metagenome assembled genomes' were recovered, corresponding to new species of Pontibacillus, several species from the Halobacteria and one new member of the Nanohaloarchaeota. The viral assemblage, although composed of the morphotypes typical of high salt systems, showed little similarity to previously isolated/reconstructed halophages. Several putative prophages of Pontibacillus and haloarchaeal hosts were identified. Remarkably, the Peñahueca sulfate-rich metagenome contained CRISPR-associated proteins and repetitions which were over 10-fold higher than in most hypersaline systems analysed so far.
Identifiants
pubmed: 31087616
doi: 10.1111/1462-2920.14680
doi:
Substances chimiques
RNA, Ribosomal, 16S
0
Sulfates
0
Sodium Chloride
451W47IQ8X
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3577-3600Subventions
Organisme : European Regional Development Fund
ID : ESP2015-69540-R
Pays : International
Organisme : European Regional Development Fund
ID : AYA2011-24803
Pays : International
Organisme : Spanish Ministry of Economy
ID : CGL2015-66455-R
Pays : International
Organisme : Spanish Ministry of Economy
ID : CLG2015_66686-C3-3
Pays : International
Organisme : Spanish Ministry of Economy
ID : CLG2015_66686-C3-1
Pays : International
Informations de copyright
© 2019 Society for Applied Microbiology and John Wiley & Sons Ltd.
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