Enrichment of cancer stem-like cells by the induction of epithelial-mesenchymal transition using lentiviral vector carrying E-cadherin shRNA in HT29 cell line.
AC133 Antigen
/ genetics
Cadherins
/ antagonists & inhibitors
Colonic Neoplasms
/ genetics
Epithelial-Mesenchymal Transition
/ genetics
Gene Expression Regulation, Neoplastic
Genetic Vectors
/ genetics
HT29 Cells
Humans
Hyaluronan Receptors
/ genetics
Lentivirus
/ genetics
Neoplasm Invasiveness
/ genetics
Neoplastic Stem Cells
/ metabolism
RNA, Small Interfering
/ genetics
E-cadherin
cancer stem cells (CSCs)
cancer therapy.
epithelial-mesenchymal transition (EMT)
lentiviral vector
Journal
Journal of cellular physiology
ISSN: 1097-4652
Titre abrégé: J Cell Physiol
Pays: United States
ID NLM: 0050222
Informations de publication
Date de publication:
12 2019
12 2019
Historique:
received:
06
02
2019
revised:
02
05
2019
accepted:
03
05
2019
pubmed:
22
5
2019
medline:
12
6
2020
entrez:
22
5
2019
Statut:
ppublish
Résumé
A better understanding of cancer stem cells (CSCs) may facilitate the prevention and treatment of cancers. Epithelial-mesenchymal transition (EMT) is a process activated during invasion and metastasis of tumors. EMT induction in normal and tumor cells makes them more resistant to chemotherapy. E-cadherin is a membrane protein and plays a role in tumor invasion, metastasis, and prognosis. Downregulation of E-cadherin is a hallmark of EMT. Here, we created a model of cancer stem-like cells enrichment via EMT induction using E-cadherin downregulation in HT29 cell line using a lentiviral vector carrying shRNA. We aimed to evaluate cancer and anti-CSC chemotherapeutics screening. The markers of EMT and CSCs were assessed and compared with control cells using flow cytometry, real-time PCR, immunocytochemistry, western blot, migration assay, invasion assay, and colony formation assay. The transduced cells showed a mesenchymal morphology. High levels of EMT-related proteins were also expressed. These results confirmed that the transduced cells underwent EMT. In addition, we observed an increased population of E-cadherin-downregulated HT29 cell line among the cells expressing colon CSC markers (CD133
Substances chimiques
AC133 Antigen
0
Cadherins
0
Hyaluronan Receptors
0
RNA, Small Interfering
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
22935-22946Informations de copyright
© 2019 Wiley Periodicals, Inc.