Identification and characterization of circular RNAs in chicken hepatocytes.
Circular RNAs
GHR
Hepatocyte
Journal
Growth hormone & IGF research : official journal of the Growth Hormone Research Society and the International IGF Research Society
ISSN: 1532-2238
Titre abrégé: Growth Horm IGF Res
Pays: Scotland
ID NLM: 9814320
Informations de publication
Date de publication:
Historique:
received:
23
08
2018
revised:
13
05
2019
accepted:
14
05
2019
pubmed:
28
5
2019
medline:
7
1
2020
entrez:
25
5
2019
Statut:
ppublish
Résumé
CircRNAs play important roles in chicken's growth. In this context, we studied the expression profiles of circRNAs between the GHR antisense transcript overexpressed and the control groups of chicken hepatocytes by using the deep RNA-sequencing technique to identify the key circRNAs involved in chicken's growth. A total of 4772 circRNAs were detected in both groups and 92 circRNAs displayed significant differential expression between two groups. Reactome pathway analysis on the parental genes of differential circRNAs indicated that most enriched and meaningful 9 pathways were related to "Mitotic G1-G1/S-M phases", and "FGFR2c ligand binding and activation". Additionally, Five exonic circRNAs were confirmed including circGHR separated from GHR. CircGHR developed in HuaiXiang chicken from d 1 to 5 w of age and was expressed at a higher level in the nucleus than in the cytoplasm. Moreover, our results specify 5 circRNAs target sites for microRNA Let-7b that is expected to target GHR mRNA, signifying their potential role in regulating GHR gene expression in chicken.
Identifiants
pubmed: 31125863
pii: S1096-6374(19)30024-3
doi: 10.1016/j.ghir.2019.05.002
pii:
doi:
Substances chimiques
Oligonucleotides, Antisense
0
RNA, Circular
0
RNA, Messenger
0
Receptors, Somatotropin
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
16-23Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.