Comparative Fluxome and Metabolome Analysis of Formate as an Auxiliary Substrate for Penicillin Production in Glucose-Limited Cultivation of Penicillium chrysogenum.


Journal

Biotechnology journal
ISSN: 1860-7314
Titre abrégé: Biotechnol J
Pays: Germany
ID NLM: 101265833

Informations de publication

Date de publication:
Oct 2019
Historique:
received: 07 01 2019
revised: 20 05 2019
pubmed: 30 5 2019
medline: 11 2 2020
entrez: 30 5 2019
Statut: ppublish

Résumé

During glucose-limited growth, a substantial input of adenosine triphosphate (ATP) is required for the production of β-lactams by the filamentous fungus Penicillium chrysogenum. Formate dehydrogenase has been confirmed in P. chrysogenum for formate oxidation allowing an extra supply of ATP, and coassimilation of glucose and formate has the potential to increase penicillin production and biomass yield. In this study, the steady-state metabolite levels and fluxes in response to cofeeding of formate as an auxiliary substrate in glucose-limited chemostat cultures at the dilution rates (D) of both 0.03 h

Identifiants

pubmed: 31140716
doi: 10.1002/biot.201900009
doi:

Substances chimiques

Formates 0
Fungal Proteins 0
Multienzyme Complexes 0
Penicillins 0
formic acid 0YIW783RG1
Hydrogenase EC 1.12.7.2
Formate Dehydrogenases EC 1.17.1.9
formate hydrogenlyase EC 1.17.1.9
Glucose IY9XDZ35W2

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

e1900009

Subventions

Organisme : the 111 Project
ID : B18022
Organisme : National key research and development program
ID : 2017ZX7402003
Organisme : the Fundamental Research Funds for the Central Universities
ID : 22221818014
Organisme : NWO-MoST Joint Program
ID : 2013DFG32630
Organisme : National Key Research and Development Program
ID : 2017ZX7402003
Organisme : 111 Project (B18022)
Organisme : Fundamental Research Funds for the Central Universities
ID : 22221818014

Informations de copyright

© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Auteurs

Guan Wang (G)

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology (ECUST), 130 Meilong Road, Shanghai, 200237, P. R. China.

Xinxin Wang (X)

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology (ECUST), 130 Meilong Road, Shanghai, 200237, P. R. China.

Tong Wang (T)

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology (ECUST), 130 Meilong Road, Shanghai, 200237, P. R. China.

Walter van Gulik (W)

Cell Systems Engineering, Department of Biotechnology, Delft University of Technology, Delft, The Netherlands.

Henk J Noorman (HJ)

DSM Biotechnology Center, Delft, The Netherlands.
Department of Biotechnology, Delft University of Technology, Delft, The Netherlands.

Yingping Zhuang (Y)

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology (ECUST), 130 Meilong Road, Shanghai, 200237, P. R. China.

Ju Chu (J)

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology (ECUST), 130 Meilong Road, Shanghai, 200237, P. R. China.

Siliang Zhang (S)

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology (ECUST), 130 Meilong Road, Shanghai, 200237, P. R. China.

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Classifications MeSH