Reducing MSH4 copy number prevents meiotic crossovers between non-homologous chromosomes in Brassica napus.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
29 05 2019
29 05 2019
Historique:
received:
19
11
2018
accepted:
11
04
2019
entrez:
31
5
2019
pubmed:
31
5
2019
medline:
18
6
2019
Statut:
epublish
Résumé
In allopolyploids, correct chromosome segregation requires suppression of non-homologous crossovers while levels of homologous crossovers are ensured. To date, no mechanism able to specifically inhibit non-homologous crossovers has been described in allopolyploids other than in bread wheat. Here, we show that reducing the number of functional copies of MSH4, an essential gene for the main crossover pathway, prevents non-homologous crossovers in allotetraploid Brassica napus. We show that non-homologous crossovers originate almost exclusively from the MSH4-dependent recombination pathway and that their numbers decrease when MSH4 returns to single copy in B. napus; by contrast, homologous crossovers remain unaffected by MSH4 duplicate loss. We also demonstrate that MSH4 systematically returns to single copy following numerous independent polyploidy events, a pattern that is probably not by chance. These results suggest that stabilization of allopolyploid meiosis can be enhanced by loss of a key meiotic recombination gene.
Identifiants
pubmed: 31142748
doi: 10.1038/s41467-019-10010-9
pii: 10.1038/s41467-019-10010-9
pmc: PMC6541637
doi:
Substances chimiques
MutS Proteins
EC 3.6.1.3
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2354Subventions
Organisme : Agence Nationale de la Recherche (French National Research Agency)
ID : ANR-14-CE19-0004 - CROC
Pays : International
Organisme : Agence Nationale de la Recherche (French National Research Agency)
ID : ANR-10-LABX-0040-SPS
Pays : International
Organisme : EC | EC Seventh Framework Programm | FP7 People: Marie-Curie Actions (FP7-PEOPLE - Specific Programme "People" Implementing the Seventh Framework Programme of the European Community for Research, Technological Development and Demonstration Activities (2007 to 2013))
ID : FP7 ITN-606956
Pays : International
Organisme : EC | EC Seventh Framework Programm | FP7 People: Marie-Curie Actions (FP7-PEOPLE - Specific Programme "People" Implementing the Seventh Framework Programme of the European Community for Research, Technological Development and Demonstration Activities (2007 to 2013))
ID : PIOF-GA-2013-628128 POLYMEIO
Pays : International
Organisme : EC | EU Framework Programme for Research and Innovation H2020 | H2020 Priority Excellent Science | H2020 Marie Skłodowska-Curie Actions (H2020 Excellent Science - Marie Skłodowska-Curie Actions)
ID : H2020 ITN-2017-765212
Pays : International
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