Type I and II PRMTs regulate catabolic as well as detoxifying processes in Aspergillus nidulans.
Aspergillus nidulans
Filamentous fungi
Posttranslational modifications
Protein arginine methyltransferase
Protein methylation
Journal
Fungal genetics and biology : FG & B
ISSN: 1096-0937
Titre abrégé: Fungal Genet Biol
Pays: United States
ID NLM: 9607601
Informations de publication
Date de publication:
08 2019
08 2019
Historique:
received:
08
07
2018
revised:
03
05
2019
accepted:
24
05
2019
pubmed:
31
5
2019
medline:
22
5
2020
entrez:
31
5
2019
Statut:
ppublish
Résumé
In filamentous fungi, arginine methylation has been implicated in morphogenesis, mycotoxin biosynthesis, pathogenicity, and stress response although the exact role of this posttranslational modification in these processes remains obscure. Here, we present the first genome-wide transcriptome analysis in filamentous fungi that compared expression levels of genes regulated by type I and type II protein arginine methyltransferases (PRMTs). In Aspergillus nidulans, three conserved type I and II PRMTs are present that catalyze asymmetric or symmetric dimethylation of arginines. We generated a double type I mutant (ΔrmtA/rmtB) and a combined type I and type II mutant (ΔrmtB/rmtC) to perform genome-wide comparison of their effects on gene expression, but also to monitor putative overlapping activities and reciprocal regulations of type I and type II PRMTs in Aspergillus. Our study demonstrates, that rmtA and rmtC as type I and type II representatives act together as repressors of proteins that are secreted into the extracellular region as the majority of up-regulated genes are mainly involved in catabolic pathways that constitute the secretome of Aspergillus. In addition to a strong up-regulation of secretory genes we found a significant enrichment of down-regulated genes involved in processes related to oxidation-reduction, transmembrane transport and secondary metabolite biosynthesis. Strikingly, nearly 50% of down-regulated genes in both double mutants correspond to redox reaction/oxidoreductase processes, a remarkable finding in light of our recently observed oxidative stress phenotypes of ΔrmtA and ΔrmtC. Finally, analysis of nuclear and cytoplasmic extracts for mono-methylated proteins revealed the presence of both, common and specific substrates of RmtA and RmtC. Thus, our data indicate that type I and II PRMTs in Aspergillus seem to co-regulate the same biological processes but also specifically affect other pathways in a non-redundant fashion.
Identifiants
pubmed: 31145992
pii: S1087-1845(19)30174-4
doi: 10.1016/j.fgb.2019.05.006
pmc: PMC6837890
mid: EMS84738
pii:
doi:
Substances chimiques
Transcription Factors
0
Protein-Arginine N-Methyltransferases
EC 2.1.1.319
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
86-100Informations de copyright
Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.
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