A modular Golden Gate toolkit for Yarrowia lipolytica synthetic biology.
Journal
Microbial biotechnology
ISSN: 1751-7915
Titre abrégé: Microb Biotechnol
Pays: United States
ID NLM: 101316335
Informations de publication
Date de publication:
11 2019
11 2019
Historique:
received:
29
11
2018
revised:
24
04
2019
accepted:
29
04
2019
pubmed:
31
5
2019
medline:
28
5
2020
entrez:
1
6
2019
Statut:
ppublish
Résumé
The oleaginous yeast Yarrowia lipolytica is an established host for the bio-based production of valuable compounds and an organism for which many genetic tools have been developed. However, to properly engineer Y. lipolytica and take full advantage of its potential, we need efficient, versatile, standardized and modular cloning tools. Here, we present a new modular Golden Gate toolkit for the one-step assembly of three transcription units that includes a selective marker and sequences for genome integration. Perfectly suited to a combinatorial approach, it contains nine different validated promoters, including inducible promoters, which allows expression to be fine-tuned. Moreover, this toolbox incorporates six different markers (three auxotrophic markers, two antibiotic-resistance markers and one metabolic marker), which allows the fast sequential construction and transformation of multiple elements. In total, the toolbox contains 64 bricks, and it has been validated and characterized using three different fluorescent reporter proteins. Additionally, it was successfully used to assemble and integrate a three-gene pathway allowing xylose utilization by Y. lipolytica. This toolbox provides a powerful new tool for rapidly engineering Y. lipolytica strains and is available to the community through Addgene.
Identifiants
pubmed: 31148366
doi: 10.1111/1751-7915.13427
pmc: PMC6801146
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1249-1259Informations de copyright
© 2019 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.
Références
ACS Synth Biol. 2015 Jul 17;4(7):824-32
pubmed: 25686303
Microb Cell Fact. 2017 Feb 17;16(1):31
pubmed: 28212656
Biotechnol Biofuels. 2018 Jan 22;11:11
pubmed: 29387172
Trends Biotechnol. 2018 Nov;36(11):1157-1170
pubmed: 30006239
Biotechnol J. 2018 Sep;13(9):e1700543
pubmed: 29377615
Biotechnol Biofuels. 2018 May 9;11:131
pubmed: 29760773
Metab Eng. 2016 Nov;38:115-124
pubmed: 27396355
Biotechnol Bioeng. 2018 Feb;115(2):464-472
pubmed: 28986998
PLoS One. 2009;4(5):e5553
pubmed: 19436741
Microb Biotechnol. 2017 Mar;10(2):450-455
pubmed: 28217858
FEMS Yeast Res. 2019 Jan 1;19(1):
pubmed: 30260383
Appl Microbiol Biotechnol. 2014 Jan;98(1):251-62
pubmed: 24136468
J Ind Microbiol Biotechnol. 2013 Nov;40(11):1273-83
pubmed: 24061566
Biotechnol Lett. 2013 Apr;35(4):571-6
pubmed: 23224822
Crit Rev Microbiol. 2014 Aug;40(3):187-206
pubmed: 23488872
Mol Biotechnol. 2018 Aug;60(8):621-635
pubmed: 29943148
Curr Genet. 1994 Jul;26(1):38-44
pubmed: 7954894
J Microbiol Methods. 2007 Sep;70(3):493-502
pubmed: 17669530
FEMS Microbiol Lett. 2019 Feb 1;366(4):
pubmed: 30698703
FEMS Yeast Res. 2015 Sep;15(6):
pubmed: 26100263
FEMS Yeast Res. 2015 Feb;15(1):1-10
pubmed: 25047958
Bioinformatics. 2014 Aug 1;30(15):2210-2
pubmed: 24728853
FEMS Yeast Res. 2017 May 1;17(3):
pubmed: 28475761
Microb Cell Fact. 2017 Jan 31;16(1):18
pubmed: 28143479
Nat Biotechnol. 2013 Aug;31(8):734-40
pubmed: 23873085
Prog Lipid Res. 2009 Nov;48(6):375-87
pubmed: 19720081
ACS Synth Biol. 2015 Sep 18;4(9):975-86
pubmed: 25871405
Appl Environ Microbiol. 2000 Aug;66(8):3283-9
pubmed: 10919782
Appl Microbiol Biotechnol. 2018 Jun;102(12):5221-5233
pubmed: 29704042
Metab Eng Commun. 2017 Oct 01;5:68-77
pubmed: 29188186
Microb Biotechnol. 2019 Nov;12(6):1249-1259
pubmed: 31148366
Methods Mol Biol. 2019;1923:153-168
pubmed: 30737739
Microb Cell Fact. 2017 Aug 15;16(1):141
pubmed: 28810867
Prog Lipid Res. 2016 Jan;61:40-50
pubmed: 26703186