RGG-motif self-association regulates eIF4G-binding translation repressor protein Scd6.
Amino Acid Motifs
Arginine
/ metabolism
Eukaryotic Initiation Factor-4G
/ chemistry
Methylation
Protein Binding
Protein Biosynthesis
Protein Multimerization
Repressor Proteins
/ metabolism
Ribonucleoproteins
/ metabolism
Saccharomyces cerevisiae
/ metabolism
Saccharomyces cerevisiae Proteins
/ metabolism
Stress, Physiological
RGG-motif proteins
Scd6 and Sbp1
Translation control
eIF4G
mRNA fate
self-association
Journal
RNA biology
ISSN: 1555-8584
Titre abrégé: RNA Biol
Pays: United States
ID NLM: 101235328
Informations de publication
Date de publication:
09 2019
09 2019
Historique:
pubmed:
4
6
2019
medline:
21
3
2020
entrez:
4
6
2019
Statut:
ppublish
Résumé
Regulation of mRNA translation plays a key role in the control of gene expression. Scd6, a conserved RGG-motif containing protein represses translation by binding to translation initiation factor eIF4G1. Here we report that Scd6 binds itself in RGG-motif dependent manner and self-association regulates its repression activity. Scd6 self-interaction competes with eIF4G1 binding and methylation of Scd6 RGG-motif by Hmt1 negatively affects self-association. Results pertaining to Sbp1 indicate that self-association could be a general feature of RGG-motif containing translation repressor proteins. Taken together, our study reveals a mechanism of regulation of eIF4G-binding RGG-motif translation repressors.
Identifiants
pubmed: 31157589
pii: 10.1080/15476286.2019.1621623
doi: 10.1080/15476286.2019.1621623
pmc: PMC6693564
doi:
Substances chimiques
Eukaryotic Initiation Factor-4G
0
Repressor Proteins
0
Ribonucleoproteins
0
Saccharomyces cerevisiae Proteins
0
Scd6 protein, S cerevisiae
0
Arginine
94ZLA3W45F
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1215-1227Subventions
Organisme : Wellcome Trust
Pays : United Kingdom
Références
J Biochem. 2010 Jun;147(6):885-93
pubmed: 20211855
J Cell Biol. 2007 Nov 5;179(3):437-49
pubmed: 17984320
Wiley Interdiscip Rev RNA. 2018 May 22;:e1479
pubmed: 29790275
Mol Cell. 2011 Sep 16;43(6):962-72
pubmed: 21925384
RNA. 2006 Apr;12(4):547-54
pubmed: 16484376
Elife. 2015 Aug 25;4:
pubmed: 26305499
Mol Cell. 2010 Sep 24;39(6):950-62
pubmed: 20864040
Am J Physiol Endocrinol Metab. 2011 Dec;301(6):E1051-64
pubmed: 21971522
J Biol Chem. 2016 Oct 21;291(43):22671-22685
pubmed: 27601476
Mol Biol Cell. 2013 Jul;24(13):2098-111
pubmed: 23676662
Mol Cell. 2007 Jun 22;26(6):795-809
pubmed: 17588515
J Biol Chem. 2019 May 3;294(18):7128-7136
pubmed: 29921587
Mol Cell Biol. 2008 Nov;28(21):6695-708
pubmed: 18765641
Nat Rev Mol Cell Biol. 2004 Oct;5(10):827-35
pubmed: 15459663
Nucleic Acids Res. 2018 Jul 2;46(W1):W329-W337
pubmed: 29860432
Philos Trans R Soc Lond B Biol Sci. 2011 Dec 27;366(1584):3638-52
pubmed: 22084390
Elife. 2018 Feb 09;7:
pubmed: 29424691
Mol Cell. 2007 Dec 14;28(5):721-9
pubmed: 18082597
Nat Struct Mol Biol. 2013 Jan;20(1):127-33
pubmed: 23222640
Plant Cell. 2009 Oct;21(10):3270-9
pubmed: 19855049
Biochem Biophys Res Commun. 1999 Jun 7;259(2):391-400
pubmed: 10362520
J Biol Chem. 2005 Sep 2;280(35):30888-98
pubmed: 15998636
Brain Res. 2018 Aug 15;1693(Pt A):24-36
pubmed: 29653083
Nucleic Acids Res. 2016 Nov 2;44(19):9358-9368
pubmed: 27613419
Cancer Res. 2012 Aug 15;72(16):3891-900
pubmed: 22850420
J Mol Biol. 2018 Nov 2;430(23):4650-4665
pubmed: 29913160
Mol Cell Biol. 2006 Jul;26(13):5120-30
pubmed: 16782896
Cell Cycle. 2012 Jul 15;11(14):2594-9
pubmed: 22767211
Cell. 2009 Feb 20;136(4):731-45
pubmed: 19239892
J Cell Biol. 2008 Nov 3;183(3):441-55
pubmed: 18981231
Biochem Soc Trans. 2015 Oct;43(5):763-72
pubmed: 26517881
J Biol Chem. 2006 Dec 29;281(52):40096-106
pubmed: 17074753
Cold Spring Harb Perspect Biol. 2012 Dec 01;4(12):
pubmed: 23209153
Cold Spring Harb Perspect Biol. 2012 Oct 01;4(10):
pubmed: 22815232
Genes Dev. 2005 Aug 15;19(16):1844-8
pubmed: 16103212
Cell. 2018 Apr 19;173(3):706-719.e13
pubmed: 29677514
Genes Dev. 2005 Jan 1;19(1):104-13
pubmed: 15630022
Mol Cell. 2018 Feb 1;69(3):465-479.e7
pubmed: 29358076
Proc Natl Acad Sci U S A. 2000 Oct 24;97(22):11910-5
pubmed: 11050225
Mol Cell. 2012 Jan 27;45(2):244-54
pubmed: 22284680
Mol Cell Biol. 2016 Aug 12;36(17):2226-35
pubmed: 27247266
BMC Genomics. 2004 Jul 16;5(1):45
pubmed: 15257761
Mol Cell. 2013 Jun 6;50(5):613-23
pubmed: 23746349
Nat Rev Mol Cell Biol. 2010 Feb;11(2):113-27
pubmed: 20094052
Cancer Res. 1993 Dec 15;53(24):5859-63
pubmed: 7503813
RNA Biol. 2012 May;9(5):610-23
pubmed: 22614839