Antimicrobial photodynamic activity of toluidine blue-carbon nanotube conjugate against Pseudomonas aeruginosa and Staphylococcus aureus - Understanding the mechanism of action.
Antimicrobial photodynamic therapy
Planktonic cells
Pseudomonas aeruginosa
Reactive oxygen species
Staphylococcus aureus
Toluidine blue multiwalled carbon nanotube
Journal
Photodiagnosis and photodynamic therapy
ISSN: 1873-1597
Titre abrégé: Photodiagnosis Photodyn Ther
Pays: Netherlands
ID NLM: 101226123
Informations de publication
Date de publication:
Sep 2019
Sep 2019
Historique:
received:
03
04
2019
revised:
09
05
2019
accepted:
17
06
2019
pubmed:
23
6
2019
medline:
11
2
2020
entrez:
23
6
2019
Statut:
ppublish
Résumé
The emergence of drug-resistant bacterial strains has raised the need to develop alternative treatment modalities to combat infectious diseases. Antimicrobial photodynamic therapy (aPDT) is an alternative to conventional treatment modalities. aPDT integrates a photosensitizer, which, after exposure to light of an appropriate wavelength, leads to the generation of cytotoxic reactive oxygen species (ROS). The aim of the present study was to synthesize a toluidine blue/multiwalled carbon nanotube conjugate (TBCNT) for enhanced photoinactivation of Pseudomonas aeruginosa and Staphylococcus aureus. Synthesized TBCNT conjugate was characterized and its antibacterial and antibiofilm activity was determined. During TBCNT synthesis, dye loading, and entrapment efficiency of the CNT were 12.04 ± 0.55% and 48.99 ± 2.33%, respectively. The photo-destruction of planktonic cells of the test bacteria was performed by exposure to a 125 mW red laser with a wavelength of 670 nm (radiant exposure of 58.49 J/cm Therefore, TBCNT conjugates may be used for the eradication of P. aeruginosa and S. aureus biofilms.
Sections du résumé
BACKGROUND
BACKGROUND
The emergence of drug-resistant bacterial strains has raised the need to develop alternative treatment modalities to combat infectious diseases. Antimicrobial photodynamic therapy (aPDT) is an alternative to conventional treatment modalities. aPDT integrates a photosensitizer, which, after exposure to light of an appropriate wavelength, leads to the generation of cytotoxic reactive oxygen species (ROS).
METHODS
METHODS
The aim of the present study was to synthesize a toluidine blue/multiwalled carbon nanotube conjugate (TBCNT) for enhanced photoinactivation of Pseudomonas aeruginosa and Staphylococcus aureus. Synthesized TBCNT conjugate was characterized and its antibacterial and antibiofilm activity was determined.
RESULTS
RESULTS
During TBCNT synthesis, dye loading, and entrapment efficiency of the CNT were 12.04 ± 0.55% and 48.99 ± 2.33%, respectively. The photo-destruction of planktonic cells of the test bacteria was performed by exposure to a 125 mW red laser with a wavelength of 670 nm (radiant exposure of 58.49 J/cm
CONCLUSION
CONCLUSIONS
Therefore, TBCNT conjugates may be used for the eradication of P. aeruginosa and S. aureus biofilms.
Identifiants
pubmed: 31228562
pii: S1572-1000(19)30164-4
doi: 10.1016/j.pdpdt.2019.06.014
pii:
doi:
Substances chimiques
Nanotubes, Carbon
0
Photosensitizing Agents
0
Reactive Oxygen Species
0
Tolonium Chloride
15XUH0X66N
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
305-316Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.